EGF R antibody | IJ01-1A12

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Anti EGF Receptor Antibody, clone IJ01-1A12 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti EGF Receptor Antibody, clone IJ01-1A12 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western Blot analysis of A431 untreated and EGF treated whole cell lysates probed with (A) Mouse anti EGF R antibody (**VMA00982**) followed by detection with HRP conjugated Goat anti Mouse IgG (H/L) (1/10,000, STAR207P) (B) Mouse anti EGF R antibody (pThr669) (VMA00983) followed by detection with HRP conjugated Goat anti Mouse IgG (H/L) (1/10,000, STAR207P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a 31.4 second exposure. EGF R has a molecular weight of 177 kDa.

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Mouse anti EGF Receptor

Product Type: PrecisionAb Monoclonal
Clone: IJ01-1A12
Isotype: IgG1
Format: Purified
Specificity: EGF R

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VMA00982 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human EGF receptor antibody recognizes the epidermal growth factor receptor, also known as Proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1.

The protein encoded by EGF R is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGF receptor is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in EGF R are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for EGF R (provided by RefSeq, Jul 2010).

Target Species: Human
Western Blotting: Mouse anti EGF R antibody detects bands of approximately 177 kDa in A431 cells
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Peptide, aa 1001-1024 (LMDEEDMDDVVDADEYLIPQQGFF) of human EGF R
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20^oC, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/10000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.