EGF R antibody | AbD30787
Human anti Human EGF R antibody recognizes the epidermal growth factor (EGF) receptor, a 150 kDa transmembrane receptor also known as ERBB1. The EGF receptor is a member of the ERBB family of four structurally related receptor tyrosine kinases. Binding of one of the many EGF receptor ligands (EGF, amphiregulin, TGFα, epiregulin) causes homodimerization of the receptor. This leads to downstream activation of several signalling pathways including MAPK/ERK, PI3K/Akt and PLCγ/PKC (Prenzel et al. 2001).
Overexpression of the EGF receptor promotes gene amplification and mutation consequence in cell proliferation, survival, invasion, metastasis, and tumor induced neoangiogenesis (Ciardiello & Tortora, 2008).
Overexpression of the EGF receptor promotes gene amplification and mutation consequence in cell proliferation, survival, invasion, metastasis, and tumor induced neoangiogenesis (Ciardiello & Tortora, 2008).
Product Details
- Target Species
- Human
- Western Blotting
- Human anti EGF R antibody detects a band of approximately 170 kDa in A431 cell lysate
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags
- Preparation
- Prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- EGF R peptide conjugated to BSA and human transferrin
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P00533
- Entrez Gene
- EGFR
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Regulatory
- For research purposes only
Applications of EGF R antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/200 | 1/2000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
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