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EGF R antibody | AbD30787

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Western Blot Validated
Anti EGF R Antibody, clone AbD30787 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti EGF R Antibody, clone AbD30787 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti EGF R Antibody, clone AbD30787 (PrecisionAb Monoclonal Antibody) gallery image 1
Western Blot analysis of MDA-MB-468 untreated and EGF treated whole cell lysates probed with (A) Human anti EGF R antibody (VMA00474) or (B) Human anti EGF R (pTyr1045) (VMA00515) antibody followed by detection with HRP conjugated Human anti Bacterial Alkaline Phosphatase (HCA275P) at a dilution of (A) 1/1000 or (B) 1/10,000. Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 24.699 second or a (B) 53.205 second exposure. EGF R (pTyr1045) has a molecular weight of 179 kDa.
Anti EGF R Antibody, clone AbD30787 (PrecisionAb Monoclonal Antibody) gallery image 2
A431 lysate (25 μl) was loaded onto a Criterion 4-15% gel (lanes 2 -6), run under reducing conditions and proteins were transferred onto a nitrocellulose membrane. Bio-Rad Precision Plus Protein Prestained Standards were run in lane 1. Human anti EGF R (VMA00474) was used to detect EGF R (approximate MW of 170 kDa) in lanes 2 - 5. HRP-conjugated Human anti Bacterial Alkaline Phosphatase (HCA275P) was used as a secondary antibody at a dilution of 1/10000 in lanes 2-6. The blot was visualized with the ChemiDoc MP Imaging System.

Human anti EGF R

Product Type
PrecisionAb Monoclonal
Clone
AbD30787
Isotype
HuCAL Fab bivalent
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00474
Datasheet
WB
MSDS
100 µl loader

Human anti Human EGF R antibody recognizes the epidermal growth factor (EGF) receptor, a 150 kDa transmembrane receptor also known as ERBB1. The EGF receptor is a member of the ERBB family of four structurally related receptor tyrosine kinases. Binding of one of the many EGF receptor ligands (EGF, amphiregulin, TGFα, epiregulin) causes homodimerization of the receptor. This leads to downstream activation of several signalling pathways including MAPK/ERK, PI3K/Akt and PLCγ/PKC (Prenzel et al. 2001).

Overexpression of the EGF receptor promotes gene amplification and mutation consequence in cell proliferation, survival, invasion, metastasis, and tumor induced neoangiogenesis (Ciardiello & Tortora, 2008).

Product Details

Target Species
Human
Western Blotting
Human anti EGF R antibody detects a band of approximately 170 kDa in A431 cell lysate
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags
Preparation
Prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
EGF R peptide conjugated to BSA and human transferrin
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P00533
Entrez Gene
EGFR
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of EGF R antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200 1/2000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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