EGF R antibody | 1E4

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Sheep anti Human EGF Receptor

Product Type
Monoclonal Antibody
Clone
1E4
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2198 E F 0.5 mg
Sheep anti Human EGF receptor antibody, clone 1E4 recognizes the human EGF receptor, also known as Proto-oncogene c-ErbB-1 or Receptor tyrosine-protein kinase erbB-1.

EGF receptor is a 1186 amino acid single pass type 1 trensmembrane glycoprotein inciolved in binding EGF and translocating it to the nucleus via endosomes ER and golgi. Sheep anti Human EGF receptor antibody, clone 1E4 may be of value in developing assays for EGF R in a range of biological samples.

Sheep anti Human EGF receptor antibody, clone 1E4 recognizes a cell surface epitope, and stains A431 cells strongly in flow cytometry.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P00533 Related reagents
Entrez Gene
EGFR Related reagents
Regulatory
For research purposes only

Applications of EGF R antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB