EEF2 antibody | AB02/1A11

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Mouse anti EEF2

Product Type
PrecisionAb Monoclonal
Clone
AB02/1A11
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00856
Datasheet
WB
SDS
100 µl loader

Mouse anti EEF2 antibody recognizes eukaryotic elongation factor 2, also known as EF2.

EEF2 plays a key role in protein synthesis, mediating the translocation step of elongation by catalyzing tRNA movement via GTP hydrolysis. When phosphorylated at Thr56, protein synthesis is disrupted, and cells use this mechanism to control translation under multiple biological conditions including cell cycle progression (Oji et al. 2014). Abnormal phosphorylation of EEF2 appears to occur in Alzheimer's Disease (Beckelman et al. 2019). EEF2 is a regulator of cardiomyocyte apoptosis during myocardial ischemia reperfusion, which happens after myocardial infarction and leads to gradual development of heart failure (Zhang et al. 2017). EEF2 is highly involved in regulation of cancer metastasis, and EEF2 expression in cells has been found to increase cancer cell migration and invasion capabilities (Cheng et al. 2018). In addition, EEF2 may be involved with promoting cell survival under conditions of starvation, hypoxia, or metabolic stress (Wang et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti EEF2 antibody detects a band of approximately 94 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 300-858 of human EEF2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P13639
Entrez Gene
EEF2
GO Terms
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0030529 ribonucleoprotein complex
GO:0003746 translation elongation factor activity
GO:0005829 cytosol
GO:0005525 GTP binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EEF2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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