eEF1A1 antibody

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Mouse anti eEF1A1

Mouse anti-human eEF1A1 antibody recognizes elongation factor 1-alpha 1, also known as CTCL tumor antigen, cervical cancer suppressor 3, elongation factor Tu, or glucocorticoid receptor AF-1 specific elongation factor.

eEF1A1 Antibody with Control Lysate

Mouse anti-human eEF1A1 antibody recognizes elongation factor 1-alpha 1, also known as CTCL tumor antigen, cervical cancer suppressor 3, elongation factor Tu, or glucocorticoid receptor AF-1 specific elongation factor.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00511 WB 100 µl
VMA00511KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human eEF1A1 antibody recognizes the elongation factor 1-alpha 1, also known as CTCL tumor antigen, cervical cancer suppressor 3, elongation factor 1 alpha subunit, elongation factor Tu, eukaryotic elongation factor 1 A-1, eukaryotic translation elongation factor 1 alpha 1-like 14, glucocorticoid receptor AF-1 specific elongation factor or leukocyte receptor cluster member 7.

The EEF1A1 gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome (Ditzel et al. 2000). EEF1A1 has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. (provided by RefSeq, Jul 2008).

Mouse anti Human eEF1A1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti eEF1A1 detects a band of approximately 50 kDa in HEK293 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli derived recombinant protein corresponding to amino acids 1-462 of human eEF1A1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P68104 Related reagents
Entrez Gene
EEF1A1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0003746 translation elongation factor activity
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0005853 eukaryotic translation elongation factor 1 complex
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of eEF1A1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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