EB1 antibody | AB03/4B6
Mouse anti Human EB1 antibody recognizes the APC-binding protein EB1, also known as adenomatous polyposis coli-binding protein EB1, end-binding protein 1 and microtubule-associated protein RP/EB family member 1.
EB1 is a highly conserved, important regulator of microtubule dynamics which stabilizes and stimulates growth at the plus-end of microtubules (Jayatilaka et al. 2018). The protein consists of two functional domains: the N-terminal CH domain recognizes the growing ends of microtubules, while the C-terminal EBH domain recruits microtubule plus-end tracking proteins (+TIPs) (van Haren et al. 2018) which also regulate microtubule dynamics and functions. Interaction between EB1 and +TIPs appears to depend on post-translational modifications of EB1. For example, phosphorylation of EB1, mediated by ASK1, contributes to the ability of EB1 to track plus-ends of microtubules (Ran et al. 2017). ASK1 promotes proliferation of pancreatic cancer cells, and EB1 phosphorylation has been found to mediate the function of ASK1 and contribute to pancreatic cancer development (Gao et al. 2017). EB1 overexpression has been identified in early stages of oral cancer (Kumar et al. 2016) and colorectal cancer (Stypula-Cyrus et al. 2014).
EB1 is a highly conserved, important regulator of microtubule dynamics which stabilizes and stimulates growth at the plus-end of microtubules (Jayatilaka et al. 2018). The protein consists of two functional domains: the N-terminal CH domain recognizes the growing ends of microtubules, while the C-terminal EBH domain recruits microtubule plus-end tracking proteins (+TIPs) (van Haren et al. 2018) which also regulate microtubule dynamics and functions. Interaction between EB1 and +TIPs appears to depend on post-translational modifications of EB1. For example, phosphorylation of EB1, mediated by ASK1, contributes to the ability of EB1 to track plus-ends of microtubules (Ran et al. 2017). ASK1 promotes proliferation of pancreatic cancer cells, and EB1 phosphorylation has been found to mediate the function of ASK1 and contribute to pancreatic cancer development (Gao et al. 2017). EB1 overexpression has been identified in early stages of oral cancer (Kumar et al. 2016) and colorectal cancer (Stypula-Cyrus et al. 2014).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti EB1 detects a band of approximately 31 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-268 of human EB1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q15691
- Entrez Gene
- MAPRE1
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0000236 mitotic prometaphase
- GO:0005829 cytosol
- GO:0005813 centrosome
- GO:0008022 protein C-terminus binding
- GO:0008283 cell proliferation
- GO:0030981 cortical microtubule cytoskeleton
- GO:0031115 negative regulation of microtubule polymerization
- GO:0035372 protein localization to microtubule
- GO:0051010 microtubule plus-end binding
- GO:0051301 cell division
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of EB1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | |||
| Western Blotting | 1/1000 | 1/2000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
