EB1 antibody | AB03/4B6

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Anti EB1 Antibody, clone AB03/4B6 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti EB1 Antibody, clone AB03/4B6 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti EB1 Antibody, clone AB03/4B6 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with EB1 antibody (1/2,000, **VMA00702**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to EB1 (molecular weight 31 kDa).

Anti EB1 Antibody, clone AB03/4B6 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of Mouse anti EB1 from HEK293 whole cell lysate. The Input lane is 5% input; the Mouse anti EB1-IP lane is the immunoprecipitation with the Mouse anti EB1 antibody (2.5 µg, **VMA00702**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using Mouse anti EB1 antibody (1/2000, **VMA00702**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10000, 1705047) and visualized on the ChemiDoc MP. Arrow points to Mouse anti EB1.

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WB IP

Mouse anti EB1

Product Type: PrecisionAb Monoclonal
Clone: AB03/4B6
Isotype: IgG1
Format: Purified
Specificity: EB1

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00702 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human EB1 antibody recognizes the APC-binding protein EB1, also known as adenomatous polyposis coli-binding protein EB1, end-binding protein 1 and microtubule-associated protein RP/EB family member 1.

EB1 is a highly conserved, important regulator of microtubule dynamics which stabilizes and stimulates growth at the plus-end of microtubules (Jayatilaka et al. 2018). The protein consists of two functional domains: the N-terminal CH domain recognizes the growing ends of microtubules, while the C-terminal EBH domain recruits microtubule plus-end tracking proteins (+TIPs) (van Haren et al. 2018) which also regulate microtubule dynamics and functions. Interaction between EB1 and +TIPs appears to depend on post-translational modifications of EB1. For example, phosphorylation of EB1, mediated by ASK1, contributes to the ability of EB1 to track plus-ends of microtubules (Ran et al. 2017). ASK1 promotes proliferation of pancreatic cancer cells, and EB1 phosphorylation has been found to mediate the function of ASK1 and contribute to pancreatic cancer development (Gao et al. 2017). EB1 overexpression has been identified in early stages of oral cancer (Kumar et al. 2016) and colorectal cancer (Stypula-Cyrus et al. 2014).

Target Species: Human
Western Blotting: Mouse anti EB1 detects a band of approximately 31 kDa in HeLa cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-268 of human EB1
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000	1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q15691
Entrez Gene: MAPRE1
GO Terms: GO:0000086 G2/M transition of mitotic cell cycle; GO:0000236 mitotic prometaphase; GO:0005829 cytosol; GO:0005813 centrosome; GO:0008022 protein C-terminus binding; GO:0008283 cell proliferation; GO:0030981 cortical microtubule cytoskeleton; GO:0031115 negative regulation of microtubule polymerization; GO:0035372 protein localization to microtubule; View More GO Terms; GO:0051010 microtubule plus-end binding; GO:0051301 cell division