EB1 antibody | AB03/4B6

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Mouse anti EB1

Product Type
PrecisionAb Monoclonal
Clone
AB03/4B6
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00702
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human EB1 antibody recognizes the APC-binding protein EB1, also known as adenomatous polyposis coli-binding protein EB1, end-binding protein 1 and microtubule-associated protein RP/EB family member 1.

EB1 is a highly conserved, important regulator of microtubule dynamics which stabilizes and stimulates growth at the plus-end of microtubules (Jayatilaka et al. 2018). The protein consists of two functional domains: the N-terminal CH domain recognizes the growing ends of microtubules, while the C-terminal EBH domain recruits microtubule plus-end tracking proteins (+TIPs) (van Haren et al. 2018) which also regulate microtubule dynamics and functions. Interaction between EB1 and +TIPs appears to depend on post-translational modifications of EB1. For example, phosphorylation of EB1, mediated by ASK1, contributes to the ability of EB1 to track plus-ends of microtubules (Ran et al. 2017). ASK1 promotes proliferation of pancreatic cancer cells, and EB1 phosphorylation has been found to mediate the function of ASK1 and contribute to pancreatic cancer development (Gao et al. 2017). EB1 overexpression has been identified in early stages of oral cancer (Kumar et al. 2016) and colorectal cancer (Stypula-Cyrus et al. 2014).

Product Details

Target Species
Human
Western Blotting
Mouse anti EB1 detects a band of approximately 31 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-268 of human EB1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q15691
Entrez Gene
MAPRE1
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000236 mitotic prometaphase
GO:0005829 cytosol
GO:0005813 centrosome
GO:0008022 protein C-terminus binding
GO:0008283 cell proliferation
GO:0030981 cortical microtubule cytoskeleton
GO:0031115 negative regulation of microtubule polymerization
GO:0035372 protein localization to microtubule
GO:0051010 microtubule plus-end binding
GO:0051301 cell division
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml