E3 UBIQUITIN-PROTEIN Ligase CBL antibody
Mouse anti E3 UBIQUITIN-PROTEIN Ligase CBL
Mouse anti-human E3 ubiquitin-protein ligase CBL antibody recognizes E3 ubiquitin-ligase CBL, also known as casitas B-lineage lymphoma proto-oncogene, proto-oncogene c-Cbl, RING finger protein 55 or signal transduction protein CBL.
E3 UBIQUITIN-PROTEIN Ligase Cbl Antibody with Control Lysate
Mouse anti-human E3 ubiquitin-protein ligase CBL antibody recognizes E3 ubiquitin-ligase CBL, also known as casitas B-lineage lymphoma proto-oncogene, proto-oncogene c-Cbl, RING finger protein 55 or signal transduction protein CBL.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
---|---|---|---|---|---|---|
VMA00510 | WB | 100 µl | ||||
VMA00510KT | WB | 2 Westerns | ||||
With Lysate | 400µg |
The CBL gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. CBL gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in CBL are also the cause of Noonan syndrome-like disorder (NSLL) (provided by RefSeq, Mar 2012).
Mouse anti Human E3 ubiquitin-protein ligase CBL antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti E3 ubiquitin-protein ligase CBL detects a band of approximately 120 kDa in K562 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E. coli derived recombinant protein corresponding to amino acids 648-906 of human E3 ubiquitin-protein ligase CBL
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P22681 Related reagents
- Entrez Gene
- CBL Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005829 cytosol
- GO:0004842 ubiquitin-protein ligase activity
- GO:0004871 signal transducer activity
- GO:0005634 nucleus
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0008270 zinc ion binding
- GO:0017124 SH3 domain binding
- GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
- GO:0048260 positive regulation of receptor-mediated endocytosis
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of E3 UBIQUITIN-PROTEIN Ligase CBL antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1000 | ||
Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
Description | Product Code | Pack Size | Applications | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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