E3 UBIQUITIN-PROTEIN Ligase CBL antibody

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Mouse anti E3 UBIQUITIN-PROTEIN Ligase CBL

Mouse anti-human E3 ubiquitin-protein ligase CBL antibody recognizes E3 ubiquitin-ligase CBL, also known as casitas B-lineage lymphoma proto-oncogene, proto-oncogene c-Cbl, RING finger protein 55 or signal transduction protein CBL.

E3 UBIQUITIN-PROTEIN Ligase Cbl Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1

Product Code	Applications	Pack Size
VMA00510	WB	100 µl
VMA00510KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human E3 ubiquitin-protein ligase CBL antibody recognizes E3 ubiquitin-ligase CBL, also known as Cas-Br-M (murine) ecotropic retroviral transforming sequence, RING finger protein 55, casitas B-lineage lymphoma proto-oncogene or proto-oncogene c-Cbl.

The CBL gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. CBL gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in CBL are also the cause of Noonan syndrome-like disorder (NSLL) (provided by RefSeq, Mar 2012).

Mouse anti Human E3 ubiquitin-protein ligase CBL antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

E. coli derived recombinant protein corresponding to amino acids 648-906 of human E3 ubiquitin-protein ligase CBL

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P22681 Related reagents
Entrez Gene: CBL Related reagents
GO Terms: GO:0005509 calcium ion binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0005829 cytosol; GO:0004842 ubiquitin-protein ligase activity; GO:0004871 signal transducer activity; GO:0005634 nucleus; GO:0007173 epidermal growth factor receptor signaling pathway; GO:0008270 zinc ion binding; GO:0017124 SH3 domain binding; GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway; GO:0048260 positive regulation of receptor-mediated endocytosis
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of E3 UBIQUITIN-PROTEIN Ligase CBL antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti E3 ubiquitin-protein ligase CBL detects a band of approximately 120 kDa in K562 cell lysate
Western Blotting: Anti E3 ubiquitin-protein ligase CBL detects a band of approximately 120 kDa in K562 cell lysate
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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