E3 UBIQUITIN-PROTEIN Ligase CBL antibody

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Mouse anti E3 UBIQUITIN-PROTEIN Ligase CBL

E3 Ubiquitin-protein ligase CBL antibody recognizes E3 ubiquitin-protein ligase CBL, also known as Cas-Br-M (murine) ecotropic retroviral transforming sequence, RING finger protein 55, or casitas B-lineage lymphoma proto-oncogene, rare, fra(11)(q23.3).

E3 UBIQUITIN-PROTEIN Ligase CBL Antibody with Control Lysate

E3 Ubiquitin-protein ligase CBL antibody recognizes E3 ubiquitin-protein ligase CBL, also known as Cas-Br-M (murine) ecotropic retroviral transforming sequence, RING finger protein 55, or casitas B-lineage lymphoma proto-oncogene, rare, fra(11)(q23.3).

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00247 WB 100 µl
VMA00247KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human E3 ubiquitin-protein ligase CBL antibody recognizes the E3 ubiquitin-protein ligase CBL, also known as Cas-Br-M (murine) ecotropic retroviral transforming sequence, E3 ubiquitin-protein ligase CBL, RING finger protein 55, casitas B-lineage lymphoma proto-oncogene, fragile site, folic acid type, rare, fra(11)(q23.3), oncogene CBL2, proto-oncogene c-Cbl and signal transduction protein CBL.

Encoded by the CBL gene, E3 ubiquitin-protein ligase CBL is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. E3 ubiquitin-protein ligase CBL is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. CBL has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in CBL are also the cause of Noonan syndrome-like disorder (provided by RefSeq, Mar 2012).

Mouse anti Human E3 ubiquitin-protein ligase CBL antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti E3 ubiquitin-protein ligase CBL detects a band of approximately 120 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human E3 ubiquitin-protein ligase CBL expressed in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P22681 Related reagents
Entrez Gene
CBL Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008270 zinc ion binding
GO:0017124 SH3 domain binding
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0048260 positive regulation of receptor-mediated endocytosis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of E3 UBIQUITIN-PROTEIN Ligase CBL antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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