Dynein Light Chain 1 Cytoplasmic antibody | AB02/2C7

Mouse anti Dynein Light Chain 1 Cytoplasmic

Product Type
PrecisionAb Monoclonal
Clone
AB02/2C7
Isotype
IgG2b
Format
Purified
Specificity
Dynein Light Chain 1 Cytoplasmic

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VMA00075
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WB 100 µl loader
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Mouse anti Human dynein light chain 1 cytoplasmic antibody recognizes dynein light chain 1 cytoplasmic, also known as 8 kDa dynein light chain, DLC8 dynein light chain LC8-type 1 and protein inhibitor of neuronal nitric oxide synthase or PIN.

DYNLL1 forms a component of dynein, a large motor protein which, fuelled by ATP, generates force on microtubules and plays roles in cell division and intracellular transport (Roberts et al. 2013). Cytoplasmic dynein consists of heavy, intermediate, light intermediate, and light chain subunits. DYNLL1 forms part of a light chain subunit, binds to the N-terminal region of intermediate chains, and can interact with a variety of non-dynein proteins such as myosin V, nNOS and Pak1, suggesting a bridge between dynein and cargo (Stuchell-Brereton et al. 2011). Independently of its role within dynein, DYNLL1 is also a likely regulator of over a hundred diverse targets (Jurado et al. 2012). DYNLL1 plays a role in a range of cellular functions, including DNA repair and maintenance of genome stability (West et al. 2019) and B cell development (King et al. 2017). Links between DYNLL1 and cancer have been established, for example, deletion of the gene encoding DYNLL1 confers resistance to PARP inhibitors in BRCA1-mutated cells (Becker et al. 2018).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti dynein light chain 1 cytoplasmic detects a band of approximately 10 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. Coli derived recombinant protein, aa 1-89 of human dynein light chain cytoplasmic
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P63167
Entrez Gene
DYNLL1
GO Terms
GO:0005886 plasma membrane
GO:0000086 G2/M transition of mitotic cell cycle
GO:0005868 cytoplasmic dynein complex
GO:0003777 microtubule motor activity
GO:0030036 actin cytoskeleton organization
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005634 nucleus
GO:0005874 microtubule
GO:0006350 transcription
GO:0006810 transport
GO:0007017 microtubule-based process
GO:0007292 female gamete generation
GO:0009653 anatomical structure morphogenesis
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0042326 negative regulation of phosphorylation
GO:0045449 regulation of transcription
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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