Dopamine Transporter antibody | DAT-Nt(4F8)
Rat anti Human dopamine transporter antibody, clone DAT-Nt recognizes the human sodium dependent dopamine transporter encoded by the SLC6A3 gene. It recognizes an epitope rear the N-terminus. Rat anti Human dopamine transporter antibody, clone DAT-Nt does not cross react with serotonin and norepinephrine transporters.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Monkey Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- None present
- N-terminus of human dopamine transporter fused to Glutathione S-transferase.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- Q01959 Related reagents
- Entrez Gene
- SLC6A3 Related reagents
- GO Terms
- GO:0042136 neurotransmitter biosynthetic process
- GO:0005887 integral to plasma membrane
- GO:0005737 cytoplasm
- GO:0008219 cell death
- GO:0030424 axon
- GO:0043025 neuronal cell body
- For research purposes only
Applications of Dopamine Transporter antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/1000||1/10 000|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Brain (caudate, putamen and nucleus accumbens).
- Western Blotting
- 0100-0075 recognises a diffuse band at approximatley 70-85 kDa on extracts from human caudate, putamen and nucleus accumbens.
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Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)||STAR131A||1 ml||C E P WB|
|Goat anti Rat IgG:Biotin (Mouse Adsorbed)||STAR131B||0.5 mg||C E IF P WB|
|Rabbit F(ab')2 anti Rat IgG:Dylight®800||STAR16D800GA||0.1 mg||F IF WB|
|Rabbit F(ab')2 anti Rat IgG:FITC||STAR17B||1 mg||F|
|Rabbit F(ab')2 anti Rat IgG:HRP||STAR21B||1 mg||C E P RE|
|Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)||STAR69||0.5 ml||F|
|Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)||STAR71D649GA||0.1 mg||F IF|
|Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)||STAR71D800GA||0.1 mg||F IF WB|
|Goat anti Rat IgG:HRP (Mouse Adsorbed)||STAR72||0.5 mg||C E P|
|Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)||STAR73||0.5 ml||F|
Product Specific References
References for Dopamine Transporter antibody
Miller, G.W. et al. (1997) Immunochemical analysis of dopamine transporter protein in Parkinson's disease.
Ann Neurol. 41 (4): 530-9.
Kwon, O.B. et al. (2008) Neuregulin-1 regulates LTP at CA1 hippocampal synapses through activation of dopamine D4 receptors.
Proc Natl Acad Sci U S A. 105 (40): 15587-92.