Dopamine Transporter antibody | DAT-Nt(4F8)

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Rat anti Human Dopamine Transporter

Product Type
Monoclonal Antibody
Clone
DAT-Nt(4F8)
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0100-0075 C IF WB 0.1 ml
Rat anti Human dopamine transporter antibody, clone DAT-Nt recognizes the human sodium dependent dopamine transporter encoded by the SLC6A3 gene. It recognizes an epitope rear the N-terminus. Rat anti Human dopamine transporter antibody, clone DAT-Nt does not cross react with serotonin and norepinephrine transporters.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Monkey
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
None present
Immunogen
N-terminus of human dopamine transporter fused to Glutathione S-transferase.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q01959 Related reagents
Entrez Gene
SLC6A3 Related reagents
GO Terms
GO:0042136 neurotransmitter biosynthetic process
GO:0005887 integral to plasma membrane
GO:0005737 cytoplasm
GO:0008219 cell death
GO:0030424 axon
GO:0043025 neuronal cell body
Regulatory
For research purposes only

Applications of Dopamine Transporter antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen 1/1000 1/10 000
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Brain (caudate, putamen and nucleus accumbens).
Western Blotting
0100-0075 recognises a diffuse band at approximatley 70-85 kDa on extracts from human caudate, putamen and nucleus accumbens.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Application Based External Images

Immunofluorescence

Product Specific References

References for Dopamine Transporter antibody

  1. Miller, G.W. et al. (1997) Immunochemical analysis of dopamine transporter protein in Parkinson's disease.
    Ann Neurol. 41 (4): 530-9.
  2. Kwon, O.B. et al. (2008) Neuregulin-1 regulates LTP at CA1 hippocampal synapses through activation of dopamine D4 receptors.
    Proc Natl Acad Sci U S A. 105 (40): 15587-92.