Rat anti Human dopamine transporter antibody, clone DAT-Nt recognizes the human sodium dependent dopamine transporter encoded by the SLC6A3 gene. It recognizes an epitope rear the N-terminus. Rat anti Human dopamine transporter antibody, clone DAT-Nt does not cross react with serotonin and norepinephrine transporters.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- None present
- N-terminus of human dopamine transporter fused to Glutathione S-transferase.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
neurotransmitter biosynthetic process
integral to plasma membrane
neuronal cell body
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Dopamine Transporter antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Brain (caudate, putamen and nucleus accumbens).
- Western Blotting
- 0100-0075 recognises a diffuse band at approximatley 70-85 kDa on extracts from human caudate, putamen and nucleus accumbens.
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Secondary Antibodies Available
Application Based External Images
Product Specific References
References for Dopamine Transporter antibody
Miller, G.W. et al. (1997) Immunochemical analysis of dopamine transporter protein in Parkinson's disease.
Ann Neurol. 41 (4): 530-9.
Kwon, O.B. et al. (2008) Neuregulin-1 regulates LTP at CA1 hippocampal synapses through activation of dopamine D4 receptors.
Proc Natl Acad Sci U S A. 105 (40): 15587-92.
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