DNA PKcs antibody

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Mouse anti DNA PKcs

Mouse anti human DNA PKcs antibody recognizes the DNA-dependent protein kinase catalytic subunit, also known as PRKDC, DNA-PK catalytic subunit or hyper-radiosensitivity of murine scid mutation, complementing 1, p460. This antibody has been validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00423 WB 100 µl
Mouse anti Human DNA PKcs antibody recognizes DNA-dependent protein kinase catalytic subunit (DNA PKcs), also known as PRKDC and DNA-PK catalytic subunit.

Encoded by the PRKDC gene, DNA PKcs functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. DNA PKcs is a member of the PI3/PI4-kinase family (provided by RefSeq, Jul 2010).

Mouse anti Human DNA PKcs antibody detects a band of >350 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti DNA PKcs detects a band of approximately >350 kDa in MOLT-4 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E.coli-derived recombinant protein corresponding to aa 3844-4127 of human DNA PKcs
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P78527 Related reagents
Entrez Gene
PRKDC Related reagents
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of DNA PKcs antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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