DNA Methyltransferase 1 antibody
Mouse anti Human DNA methyltransferase 1 antibody recognizes the DNA (cytosine-5)-methyltransferase 1 , also known as Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI or M.HsaI MCMT.
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for DNMT1 (provided by RefSeq, Aug 2008).
Mouse anti Human DNA methyltransferase 1 antibody detects a band of 180 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for DNMT1 (provided by RefSeq, Aug 2008).
Mouse anti Human DNA methyltransferase 1 antibody detects a band of 180 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti DNA methyltransferase 1 detects a band of approximately 205 kDa in Jurkat cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant protein corresponding to amino acids 1448-1594 of human DNA methyltransferase 1 expressed in E. coli
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P26358
- Entrez Gene
- DNMT1
- GO Terms
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0003677 DNA binding
- GO:0003886 DNA (cytosine-5-)-methyltransferase activity
- GO:0005634 nucleus
- GO:0006350 transcription
- GO:0008134 transcription factor binding
- GO:0010216 maintenance of DNA methylation
- GO:0010628 positive regulation of gene expression
- GO:0016568 chromatin modification
- GO:0051571 positive regulation of histone H3-K4 methylation
- GO:0051573 negative regulation of histone H3-K9 methylation
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of DNA Methyltransferase 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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