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DNA Methyltransferase 1 antibody

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Western Blot Validated
Anti DNA Methyltransferase 1 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with DNA methyltransferase 1 antibody (VMA00245) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 183 second exposure. Arrow points to DNA methyltransferase 1 (molecular weight 205 kDa).

Mouse anti DNA Methyltransferase 1

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00245
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human DNA methyltransferase 1 antibody recognizes the DNA (cytosine-5)-methyltransferase 1 , also known as Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI or M.HsaI MCMT.

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for DNMT1 (provided by RefSeq, Aug 2008).

Mouse anti Human DNA methyltransferase 1 antibody detects a band of 180 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti DNA methyltransferase 1 detects a band of approximately 205 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein corresponding to amino acids 1448-1594 of human DNA methyltransferase 1 expressed in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P26358
Entrez Gene
DNMT1
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0003677 DNA binding
GO:0003886 DNA (cytosine-5-)-methyltransferase activity
GO:0005634 nucleus
GO:0006350 transcription
GO:0008134 transcription factor binding
GO:0010216 maintenance of DNA methylation
GO:0010628 positive regulation of gene expression
GO:0016568 chromatin modification
GO:0051571 positive regulation of histone H3-K4 methylation
GO:0051573 negative regulation of histone H3-K9 methylation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of DNA Methyltransferase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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