DNA Methyltransferase 1 antibody

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Mouse anti DNA Methyltransferase 1

DNA methyltransferase 1 antibody recognizes DNA (cytosine-5)-methyltransferase 1 , also known as DNMt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI or M.HsaI MCMT. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00245 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human DNA methyltransferase 1 antibody recognizes the DNA (cytosine-5)-methyltransferase 1 , also known as Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI or M.HsaI MCMT.

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for DNMT1 (provided by RefSeq, Aug 2008).

Mouse anti Human DNA methyltransferase 1 antibody detects a band of 180 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti DNA methyltransferase 1 detects a band of approximately 205 kDa in Jurkat cell lysates
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant protein corresponding to amino acids 1448-1594 of human DNA methyltransferase 1 expressed in E. coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: P26358 Related reagents
Entrez Gene: DNMT1 Related reagents
GO Terms: GO:0000122 negative regulation of transcription from RNA polymerase II promoter; GO:0003677 DNA binding; GO:0003886 DNA (cytosine-5-)-methyltransferase activity; GO:0005634 nucleus; GO:0006350 transcription; GO:0008134 transcription factor binding; GO:0010216 maintenance of DNA methylation; GO:0010628 positive regulation of gene expression; GO:0016568 chromatin modification; GO:0051571 positive regulation of histone H3-K4 methylation; GO:0051573 negative regulation of histone H3-K9 methylation
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of DNA Methyltransferase 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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