Dishevelled 2 antibody | OTI7C7

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Mouse anti Dishevelled 2

Mouse anti human dishevelled 2 antibody recognizes dishevelled segment polarity protein 2 also known as dishevelled homolog DVL-2 (homologous to Drosophila dsh), or segment polarity protein dishevelled homolog DVL-2. This antibody has been validated for western blotting using whole cell lysates.

Dishevelled 2 Antibody with Control Lysate

Mouse anti human dishevelled 2 antibody recognizes dishevelled segment polarity protein 2 also known as dishevelled homolog DVL-2 (homologous to Drosophila dsh), dsh homolog 2 or segment polarity protein dishevelled homolog DVL-2.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI7C7
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00375 WB 100 µl
VMA00375KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human dishevelled 2 antibody recognizes dishevelled 2 also known as dishevelled homolog DVL-2 (homologous to Drosophila dsh), dsh homolog 2 and segment polarity protein dishevelled homolog DVL-2.

The DVL2 gene encodes a 90 kDa protein that undergoes posttranslational phosphorylation to form a 95 kDa cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins.. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh (provided by RefSeq, Jul 2008).

Mouse anti Human dishevelled 2 antibody detects a band of 90-95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti dishevelled 2 detects a band of approximately 90-95 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 1-320 of human dishevelled 2 (NP_004413) produced in E.coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O14641 Related reagents
Entrez Gene
DVL2 Related reagents
GO Terms
GO:0001843 neural tube closure
GO:0001934 positive regulation of protein phosphorylation
GO:0004871 signal transducer activity
GO:0005109 frizzled binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006366 transcription from RNA polymerase II promoter
GO:0007379 segment specification
GO:0007507 heart development
GO:0042802 identical protein binding
GO:0021766 hippocampus development
GO:0021987 cerebral cortex development
GO:0043507 positive regulation of JUN kinase activity
GO:0044340 canonical Wnt receptor signaling pathway involved in regulation of cell proliferation
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0051091 positive regulation of transcription factor activity
GO:0060071 Wnt receptor signaling pathway, planar cell polarity pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Dishevelled 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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