Diacylglycerol Kinase Alpha antibody

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Mouse anti Diacylglycerol Kinase Alpha

Mouse anti-human diacylglycerol kinase alpha antibody recognizes diacylglycerol kinase alpha, also known as 80 kDa diacylglycerol kinase or DAG kinase alpha. This antibody has been extensively validated for western blotting using whole cell lysates.

Diacylglycerol Kinase Alpha Antibody with Control Lysate

Mouse anti-human diacylglycerol kinase alpha antibody recognizes diacylglycerol kinase alpha, also known as 80 kDa diacylglycerol kinase or DAG kinase alpha. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00496 WB 100 µl
VMA00496KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human diacylglycerol kinase alpha antibody recognizes diacylglycerol kinase alpha (DGKA), also known as 80 kDa diacylglycerol kinase or DAG kinase alpha.

The protein encoded by DGKA belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. (provided by RefSeq, Jul 2008).

Mouse anti Human diacylglycerol kinase alpha antibody detects a band of 83 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Full length recombinant human diacylglycerol kinase alpha (DGKA, NP_001336) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P23743 Related reagents
Entrez Gene
DGKA Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005509 calcium ion binding
GO:0004143 diacylglycerol kinase activity
GO:0035556 intracellular signal transduction
GO:0005524 ATP binding
GO:0007205 activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway
GO:0030168 platelet activation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Diacylglycerol Kinase Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti diacylglycerol kinase alpha detects a band of approximately 83 kDa in Jurkat cell lysate
Western Blotting
Anti diacylglycerol kinase alpha detects a band of approximately 83 kDa in Jurkat cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB