Desmoglein 1 antibody | 27B2

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Mouse anti Human Desmoglein 1

Product Type: Monoclonal Antibody
Clone: 27B2
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2271	C IF IP P WB			0.2 mg
MCA2271T	C IF IP P WB			25 µg

Mouse anti Human desmoglein 1 antibody, clone 27B2 recognises human desmoglein 1, also known as Cadherin family member 4, Desmosomal glycoprotein 1, DG1 or Pemphigus foliaceus antigen. Desmoglein 1 is a 999 amino acid mature peptide with an an additional 26aa propeptide region and a 23 aa signal peptide. Desmoglein 1 is a ~165 kDa single pass type I transmembrane glycoprotein and core component of the desmosome cell-cell junction between epithelial cells.

Desmoglein 1 is synthesized in the suprabasal layer of the skin, and is vital to epidermal integrity.

Mouse anti Human desmoglein 1 antibody, clone 27B2 does not recognise desmoglein-2 or desmoglein-3.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Recombinant human desmoglein 1.

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: Q02413 Related reagents
Entrez Gene: DSG1 Related reagents
GO Terms: GO:0005509 calcium ion binding; GO:0016021 integral to membrane; GO:0005829 cytosol; GO:0006921 cellular component disassembly involved in apoptosis; GO:0007043 cell-cell junction assembly; GO:0007156 homophilic cell adhesion; GO:0016339 calcium-dependent cell-cell adhesion; GO:0009898 internal side of plasma membrane; GO:0015643 toxin binding; GO:0050821 protein stabilization; GO:0030057 desmosome; GO:0045295 gamma-catenin binding
Regulatory: For research purposes only

Applications of Desmoglein 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting	1/100	1/1000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Skin
Western Blotting: MCA2271 detects a band of approximately 165kDa in epithelial cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Desmoglein 1 antibody

Wahl, J.K. 3rd (2002) Generation of monoclonal antibodies specific for desmoglein family members.
Hybrid Hybridomics. 21 (1): 37-44.
Mahoney, M.G. et al. (2010) Compound heterozygous desmoplakin mutations result in a phenotype with a combination of myocardial, skin, hair, and enamel abnormalities.
J Invest Dermatol. 130 (4): 968-78.
Biedermann, T. et al. (2010) Human eccrine sweat gland cells can reconstitute a stratified epidermis.
J Invest Dermatol. 130 (8): 1996-2009.
Wu, H. et al. (2000) Protection against pemphigus foliaceus by desmoglein 3 in neonates.
N Engl J Med. 343 (1): 31-5.
Oktarina, D.A. et al. (2011) IgG-induced clustering of desmogleins 1 and 3 in skin of patients with pemphigus fits with the desmoglein nonassembly depletion hypothesis.
Br J Dermatol. 165 (3): 552-62.

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