Desmoglein 1 antibody | 27B2
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Mouse anti Human desmoglein 1 antibody, clone 27B2 recognises human desmoglein 1, also known as Cadherin family member 4, Desmosomal glycoprotein 1, DG1 or Pemphigus foliaceus antigen. Desmoglein 1 is a 999 amino acid mature peptide with an an additional 26aa propeptide region and a 23 aa signal peptide. Desmoglein 1 is a ~165 kDa single pass type I transmembrane glycoprotein and core component of the desmosome cell-cell junction between epithelial cells.
Desmoglein 1 is synthesized in the suprabasal layer of the skin, and is vital to epidermal integrity.
Mouse anti Human desmoglein 1 antibody, clone 27B2 does not recognise desmoglein-2 or desmoglein-3.
Desmoglein 1 is synthesized in the suprabasal layer of the skin, and is vital to epidermal integrity.
Mouse anti Human desmoglein 1 antibody, clone 27B2 does not recognise desmoglein-2 or desmoglein-3.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Recombinant human desmoglein 1.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- Q02413 Related reagents
- Entrez Gene
- DSG1 Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0005829 cytosol
- GO:0006921 cellular component disassembly involved in apoptosis
- GO:0007043 cell-cell junction assembly
- GO:0007156 homophilic cell adhesion
- GO:0016339 calcium-dependent cell-cell adhesion
- GO:0009898 internal side of plasma membrane
- GO:0015643 toxin binding
- GO:0050821 protein stabilization
- GO:0030057 desmosome
- GO:0045295 gamma-catenin binding
- Regulatory
- For research purposes only
Applications of Desmoglein 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |||
| Western Blotting | 1/100 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Skin
- Western Blotting
- MCA2271 detects a band of approximately 165kDa in epithelial cell lysates.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Desmoglein 1 antibody
-
Wahl, J.K. 3rd (2002) Generation of monoclonal antibodies specific for desmoglein family members.
Hybrid Hybridomics. 21 (1): 37-44. -
Mahoney, M.G. et al. (2010) Compound heterozygous desmoplakin mutations result in a phenotype with a combination of myocardial, skin, hair, and enamel abnormalities.
J Invest Dermatol. 130 (4): 968-78. -
Biedermann, T. et al. (2010) Human eccrine sweat gland cells can reconstitute a stratified epidermis.
J Invest Dermatol. 130 (8): 1996-2009. -
Wu, H. et al. (2000) Protection against pemphigus foliaceus by desmoglein 3 in neonates.
N Engl J Med. 343 (1): 31-5. -
Oktarina, D.A. et al. (2011) IgG-induced clustering of desmogleins 1 and 3 in skin of patients with pemphigus fits with the desmoglein nonassembly depletion hypothesis.
Br J Dermatol. 165 (3): 552-62.
