Desmoglein 1 antibody | 27B2
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Mouse anti Human Desmoglein 1
- Product Type
- Monoclonal Antibody
- Clone
- 27B2
- Isotype
- IgG1
- Specificity
- Desmoglein 1
Antibody Quality and 
Unrivaled 
| Mouse anti Human desmoglein 1 antibody, clone 27B2 recognizes human desmoglein 1, also known as Cadherin family member 4, Desmosomal glycoprotein 1, DG1 or Pemphigus foliaceus antigen. Desmoglein 1 is a 999 amino acid mature peptide with an an additional 26aa propeptide region and a 23 aa signal peptide. Desmoglein 1 is a ~165 kDa single pass type I transmembrane glycoprotein and core component of the desmosome cell-cell junction between epithelial cells. Desmoglein 1 is expressed in the suprabasal layer of the skin and is vital to epidermal integrity (Kottke et al. 2006, Biedermann et al. 2010). Mouse anti Human desmoglein 1 antibody, clone 27B2 does not recognize desmoglein-2 or desmoglein-3. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant human desmoglein 1.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin | |
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| Immunoprecipitation | |
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| Western Blotting | |
1/100 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Skin
- Western Blotting
- MCA2271 detects a band of approximately 165kDa in epithelial cell lysates.
References for Desmoglein 1 antibody
-
Wahl, J.K. 3rd (2002) Generation of monoclonal antibodies specific for desmoglein family members.
Hybrid Hybridomics. 21 (1): 37-44. -
Mahoney, M.G. et al. (2010) Compound heterozygous desmoplakin mutations result in a phenotype with a combination of myocardial, skin, hair, and enamel abnormalities.
J Invest Dermatol. 130 (4): 968-78. -
Wu, H. et al. (2000) Protection against pemphigus foliaceus by desmoglein 3 in neonates.
N Engl J Med. 343 (1): 31-5. -
Oktarina, D.A. et al. (2011) IgG-induced clustering of desmogleins 1 and 3 in skin of patients with pemphigus fits with the desmoglein nonassembly depletion hypothesis.
Br J Dermatol. 165 (3): 552-62.
- RRID
- AB_2093299
- UniProt
- Q02413
- Entrez Gene
- DSG1
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0005829 cytosol
- GO:0006921 cellular component disassembly involved in apoptosis
- GO:0007043 cell-cell junction assembly
- GO:0007156 homophilic cell adhesion
- GO:0016339 calcium-dependent cell-cell adhesion
- GO:0009898 internal side of plasma membrane
- GO:0015643 toxin binding
- View More GO Terms
- GO:0050821 protein stabilization
- GO:0030057 desmosome
- GO:0045295 gamma-catenin binding
View more products with DESMOGLEIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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