Desmoglein 1 antibody | 27B2

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Mouse anti Human Desmoglein 1

Product Type
Monoclonal Antibody
Clone
27B2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2271T C IF IP P WB 25 µg
MCA2271 C IF IP P WB 0.2 mg
Mouse anti Human desmoglein 1 antibody, clone 27B2 recognises human desmoglein 1, also known as Cadherin family member 4, Desmosomal glycoprotein 1, DG1 or Pemphigus foliaceus antigen. Desmoglein 1 is a 999 amino acid mature peptide with an an additional 26aa propeptide region and a 23 aa signal peptide. Desmoglein 1 is a ~165 kDa single pass type I transmembrane glycoprotein and core component of the desmosome cell-cell junction between epithelial cells.

Desmoglein 1 is synthesized in the suprabasal layer of the skin, and is vital to epidermal integrity.

Mouse anti Human desmoglein 1 antibody, clone 27B2 does not recognise desmoglein-2 or desmoglein-3.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant human desmoglein 1.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q02413 Related reagents
Entrez Gene
DSG1 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0005829 cytosol
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007043 cell-cell junction assembly
GO:0007156 homophilic cell adhesion
GO:0016339 calcium-dependent cell-cell adhesion
GO:0009898 internal side of plasma membrane
GO:0015643 toxin binding
GO:0050821 protein stabilization
GO:0030057 desmosome
GO:0045295 gamma-catenin binding
Regulatory
For research purposes only

Applications of Desmoglein 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Skin
Western Blotting
MCA2271 detects a band of approximately 165kDa in epithelial cell lysates.

Product Specific References

References for Desmoglein 1 antibody

  1. Wahl, J.K. 3rd (2002) Generation of monoclonal antibodies specific for desmoglein family members.
    Hybrid Hybridomics. 21 (1): 37-44.
  2. Mahoney, M.G. et al. (2010) Compound heterozygous desmoplakin mutations result in a phenotype with a combination of myocardial, skin, hair, and enamel abnormalities.
    J Invest Dermatol. 130 (4): 968-78.
  3. Biedermann, T. et al. (2010) Human eccrine sweat gland cells can reconstitute a stratified epidermis.
    J Invest Dermatol. 130 (8): 1996-2009.
  4. Wu, H. et al. (2000) Protection against pemphigus foliaceus by desmoglein 3 in neonates.
    N Engl J Med. 343 (1): 31-5.
  5. Oktarina, D.A. et al. (2011) IgG-induced clustering of desmogleins 1 and 3 in skin of patients with pemphigus fits with the desmoglein nonassembly depletion hypothesis.
    Br J Dermatol. 165 (3): 552-62.