Dendritic Cells/B Cells antibody | RFD1

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Mouse anti Human Dendritic Cells

Product Type
Monoclonal Antibody
Clone
RFD1
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1121 C* 0.2 mg
Mouse anti Human dendritic cells antibody, clone RFD1 recognizes an epitope within the class II MHC complex. In normal tissue mouse anti human dendritic cells antibody, clone RFD1 identifies dendritic cells and a proportion of B lymphocytes. Within the macrophage/dendritic cell populations the epitope seen by RFD1 is co-expressed with RFD7 by a subset of cells that exhibit suppressive activity on T cell stimulation.Mouse anti Human dendritic cells antibody, clone RFD1 does not bind to monocytes or cells of the granulocyte lineage.

Mouse anti Human dendritic cells antibody, clone RFD1 has been used for the induction of apoptosis via MHC Class II cross linking of gastric mucosa epithelial cells (Fan et al. 1998).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Macaque
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM- liquid
Preparation
Purified IgM prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgM concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Dendritic Cells/B Cells antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Application Based External Images

Functional Assays

Product Specific References

References for Dendritic Cells/B Cells antibody

  1. Poulter, L.W. et al. (1986) Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies.
    Scand J Immunol. 24 (3): 351-7.
  2. Eckstein, A.K. et al. (2004) Thyroid associated ophthalmopathy: evidence for CD4(+) gammadelta T cells; de novo differentiation of RFD7(+) macrophages, but not of RFD1(+) dendritic cells; and loss of gammadelta and alphabeta T cell receptor expression.
    Br J Ophthalmol. 88: 803-8.
  3. Hamilton, R.F. Jr. et al. (2004) Alveolar macrophages from systemic sclerosis patients: evidence for IL-4-mediated phenotype changes.
    Am J Physiol Lung Cell Mol Physiol. 286: L1202-9.
  4. Rugtveit, J. et al. (1997) Differential distribution of B7.1 (CD80) and B7.2 (CD86) costimulatory molecules on mucosal macrophage subsets in human inflammatory bowel disease (IBD).
    Clin Exp Immunol. 110 (1): 104-13.
  5. Leonard, C.T. et al. (2000) Dendritic cells and macrophages in lung allografts: A role in chronic rejection?
    Am J Respir Crit Care Med. 161: 1349-54.
  6. Fan, X. et al. (1998) The effect of class II major histocompatibility complex expression on adherence of Helicobacter pylori and induction of apoptosis in gastric epithelial cells: a mechanism for T helper cell type 1-mediated damage.
    J Exp Med. 187: 1659-69.
  7. Figueroa, D.J. et al. (2000) Expression of the type I diabetes-associated gene LRP5 in macrophages, vitamin A system cells, and the Islets of Langerhans suggests multiple potential roles in diabetes.
    J Histochem Cytochem. 48: 1357-68.
  8. Kiekens, R.C. et al. (2001) Heterogeneity within tissue-specific macrophage and dendritic cell populations during cutaneous inflammation in atopic dermatitis.
    Br J Dermatol. 145: 957-65.
  9. Sprinzl, G.M. et al. (1996) Analysis of antigen presenting cells in the human tonsil: A clinical and histopathological study
    212, Session G, Podium
  10. Pantelidis, P. et al. (2001) Tumour necrosis factor-alpha production in fibrosing alveolitis is macrophage subset specific.
    Respir Res. 2: 365-72.
  11. Li, T.F. et al. (2002) Dendritic cells in rheumatoid synovial membrane after total removal of the hyaline articular cartilage.
    Rheumatology (Oxford). 41: 319-23.
  12. Bland, D.A. et al. (2006) H. pylori receptor MHC class II contributes to the dynamic gastric epithelial apoptotic response.
    World J Gastroenterol. 12: 5306-10.
  13. Fan, X. et al. (2000) Helicobacter pylori urease binds to class II MHC on gastric epithelial cells and induces their apoptosis.
    J Immunol. 165: 1918-24.