Dek antibody | AB02/2G4

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Mouse anti Dek

Product Type
PrecisionAb Monoclonal
Clone
AB02/2G4
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00892
Datasheet
WB
SDS
100 µl loader

Mouse anti DEK antibody recognizes protein DEK.

DEK is a highly-conserved nuclear protein involved with DNA replication, chromatin remodeling, maintenance of chromatin architecture, and mRNA splicing. The gene encoding DEK is an oncogene, and depending on experimental system, loss of DEK has been found to attenuate cell proliferation, cell survival, and chemoresistance (Smith et al. 2017). Accordingly, DEK overexpression is associated with carcinoma cell proliferation and is observed in several solid tumors including breast cancer, melanoma, bladder cancer and retinoblastoma (Çalışkaner et al. 2017). DEK is a regulator of hematopoesis, controlling numbers and fate decisions of hematopoetic stem and progenitor cells. Intracellular concentrations of DEK are regulated by inflammatory stimuli, and DEK regulates inflammation-induced NF-kappaB activation (Capitano et al. 2017).

Product Details

Target Species
Human
Western Blotting
Mouse anti DEK antibody detects a band of approximately 54 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 110-130 of human DEK
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P35659
Entrez Gene
DEK
GO Terms
GO:0003677 DNA binding
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0042393 histone binding
GO:0007165 signal transduction
GO:0005634 nucleus
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0019079 viral genome replication
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Dek antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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