Defensin antibody | DEF-3

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Mouse anti Human Defensin

Product Type
Monoclonal Antibody
Clone
DEF-3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1465T C F* P* WB 25 µg
Mouse anti Human defensin, clone DEF-3 recognizes the product of the human defensin alpha 1 gene, HNP-1 or neutrophil defensin 1, a 94 amino acid (including a 19aa signal peptide) member of the antibiotic alpha defensin family. Defensin alpha 1 is expressed by mature neutrophils.

Defensins are a group of small cyclic peptides which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.

Mouse anti Human defensin, clone DEF-3 recognizes alpha defensins (human neutrophil peptides) 1-3. Binding to defensin 4 has not been tested. Defensins are up-regulated during the acute phase of diseases such as shigellosis or infection with Helicobacter pylori (Kocsis et al. 2009)

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P59665 Related reagents
P59666 Related reagents
P12838 Related reagents
Entrez Gene
DEFA4 Related reagents
DEFA1 Related reagents
DEFA3 Related reagents
GO Terms
GO:0042742 defense response to bacterium
GO:0050832 defense response to fungus
GO:0005615 extracellular space
GO:0031640 killing of cells of other organism
GO:0009615 response to virus
GO:0006935 chemotaxis
GO:0006955 immune response
Regulatory
For research purposes only

Applications of Defensin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
Immunohistology - Frozen 0.1 0.5ug/ml
Immunohistology - Paraffin 2 0.2 1.0ug/ml
Western Blotting
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
  2. 2 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for Defensin antibody

  1. Dudal, S. et al. (2006) Release of LL-37 by activated human Vgamma9Vdelta2 T cells: a microbicidal weapon against Brucella suis.
    J Immunol. 177: 5533-9.
  2. Tschopp, C.M. et al. (2006) Granzyme B, a novel mediator of allergic inflammation: its induction and release in blood basophils and human asthma.
    Blood. 108: 2290-9.
  3. Qadri, F. et al. (2004) Acute dehydrating disease caused by Vibrio cholerae serogroups O1 and O139 induce increases in innate cells and inflammatory mediators at the mucosal surface of the gut.
    Gut. 53: 62-9.
  4. Sun, L. et al. (2005) Human beta-defensins suppress human immunodeficiency virus infection: potential role in mucosal protection.
    J Virol. 79: 14318-29.
  5. Xu, N. et al. (2008) Human alpha-defensin-1 inhibits growth of human lung adenocarcinoma xenograft in nude mice.
    Mol Cancer Ther. 7: 1588-97.
  6. Soylu, O.B. et al. (2008) Alpha-defensin expression in the gastric tissue of children with Helicobacter pylori-associated chronic gastritis: an immunohistochemical study.
    J Pediatr Gastroenterol Nutr. 46: 474-7.
  7. Raqib, R. et al. (2003) Persistence of mucosal mast cells and eosinophils in Shigella-infected children.
    Infect Immun. 71: 2684-92.
  8. Kocsis, A.K. et al. (2009) Helicobacter pylori induces the release of alpha-defensin by human granulocytes.
    Inflamm Res. 58: 241-7.
  9. Cardot-Martin, E. et al. (2015) α-Defensins partially protect human neutrophils against Panton-Valentine leukocidin produced by Staphylococcus aureus.
    Lett Appl Microbiol. 61 (2): 158-64.

Further Reading

  1. Müller, C.A. et al. (2002) Human alpha-defensins HNPs-1, -2, and -3 in renal cell carcinoma: influences on tumor cell proliferation.
    Am J Pathol. 160 (4): 1311-24.