Defensin antibody | DEF-3
Defensins are a group of small cyclic peptides which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.
Mouse anti Human defensin, clone DEF-3 recognizes alpha defensins (human neutrophil peptides) 1-3. Binding to defensin 4 has not been tested. Defensins are up-regulated during the acute phase of diseases such as shigellosis or infection with Helicobacter pylori (Kocsis et al. 2009)
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
- GO:0042742 defense response to bacterium
- GO:0050832 defense response to fungus
- GO:0005615 extracellular space
- GO:0031640 killing of cells of other organism
- GO:0009615 response to virus
- GO:0006935 chemotaxis
- GO:0006955 immune response
- For research purposes only
Applications of Defensin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1|
|Immunohistology - Frozen||0.1||0.5ug/ml|
|Immunohistology - Paraffin 2||0.2||1.0ug/ml|
- 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
- 2 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
- Histology Positive Control Tissue
- Human tonsil
Secondary Antibodies Available
Negative Isotype Controls Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Mouse IgG1 Negative Control||MCA928||F||100 Tests|
Product Specific References
References for Defensin antibody
Dudal, S. et al. (2006) Release of LL-37 by activated human Vgamma9Vdelta2 T cells: a microbicidal weapon against Brucella suis.
J Immunol. 177: 5533-9.
Tschopp, C.M. et al. (2006) Granzyme B, a novel mediator of allergic inflammation: its induction and release in blood basophils and human asthma.
Blood. 108: 2290-9.
Qadri, F. et al. (2004) Acute dehydrating disease caused by Vibrio cholerae serogroups O1 and O139 induce increases in innate cells and inflammatory mediators at the mucosal surface of the gut.
Gut. 53: 62-9.
Sun, L. et al. (2005) Human beta-defensins suppress human immunodeficiency virus infection: potential role in mucosal protection.
J Virol. 79: 14318-29.
Xu, N. et al. (2008) Human alpha-defensin-1 inhibits growth of human lung adenocarcinoma xenograft in nude mice.
Mol Cancer Ther. 7: 1588-97.
Soylu, O.B. et al. (2008) Alpha-defensin expression in the gastric tissue of children with Helicobacter pylori-associated chronic gastritis: an immunohistochemical study.
J Pediatr Gastroenterol Nutr. 46: 474-7.
Raqib, R. et al. (2003) Persistence of mucosal mast cells and eosinophils in Shigella-infected children.
Infect Immun. 71: 2684-92.
Kocsis, A.K. et al. (2009) Helicobacter pylori induces the release of alpha-defensin by human granulocytes.
Inflamm Res. 58: 241-7.
Cardot-Martin, E. et al. (2015) α-Defensins partially protect human neutrophils against Panton-Valentine leukocidin produced by Staphylococcus aureus.
Lett Appl Microbiol. 61 (2): 158-64.
Müller, C.A. et al. (2002) Human alpha-defensins HNPs-1, -2, and -3 in renal cell carcinoma: influences on tumor cell proliferation.
Am J Pathol. 160 (4): 1311-24.