DDX58 antibody

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Human anti DDX58

DDX58 antibody recognizes DEAD box protein 58, also known as the Probable ATP-dependent RNA helicase DDX58, DEAD/H box polypeptide, RNA helicase RIG-I.

Product Type
PrecisionAb™ Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00635 WB 100 µl
Human anti Human DDX58 antibody recognizes DEAD box protein 58, also known as the probable ATP-dependent RNA helicase DDX58, DEAD/H box polypeptide and RNA helicase RIG-I.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. DDX58 encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response (provided by RefSeq, Jul 2008).

Human anti Human DDX58 antibody detects a band of 102 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Human anti DDX58 antibody detects a band of approximately 102 kDa in THP-1 cells stimulated for 16 hrs with lipopolysaccharide (LPS)
Product Form
Purified IgG - liquid
Preparation
Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human DDX58
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O95786 Related reagents
Entrez Gene
DDX58 Related reagents
GO Terms
GO:0005515 protein binding
GO:0002230 positive regulation of defense response to virus by host
GO:0003723 RNA binding
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0008026 ATP-dependent helicase activity
GO:0008270 zinc ion binding
GO:0032480 negative regulation of type I interferon production
GO:0032727 positive regulation of interferon-alpha production
GO:0032728 positive regulation of interferon-beta production
GO:0045087 innate immune response
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of DDX58 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB

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