DAXX antibody

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Mouse anti DAXX

DAXX antibody recognizes death domain-associated protein 6, also known as CENP-C binding protein, ETS1-associated protein 1 or Fas-binding protein. This antibody has been extensively validated for western blotting using whole cell lysates.

DAXX Antibody with Control Lysate

DAXX antibody recognizes death domain-associated protein 6, also known as CENP-C binding protein, ETS1-associated protein 1 or Fas-binding protein. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00318 WB 100 µl
VMA00318KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human DAXX antibody recognizes the death domain-associated protein 6, also known as CENP-C binding protein, ETS1-associated protein 1, Fas-binding protein, death-associated protein 6 and fas death domain-associated protein.

Encoded by the DAXX gene, death domain-associated protein 6 is a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants (provided by RefSeq, Nov 2008).

Mouse anti Human DAXX antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti DAXX detects a band of approximately 110 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human DAXX expressed in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9UER7 Related reagents
Entrez Gene
DAXX Related reagents
GO Terms
GO:0000775 chromosome, centromeric region
GO:0002039 p53 binding
GO:0006915 apoptosis
GO:0005057 receptor signaling protein activity
GO:0005730 nucleolus
GO:0006350 transcription
GO:0007257 activation of JUN kinase activity
GO:0008134 transcription factor binding
GO:0008625 induction of apoptosis via death domain receptors
GO:0016481 negative regulation of transcription
GO:0016605 PML body
GO:0031072 heat shock protein binding
GO:0030521 androgen receptor signaling pathway
GO:0031396 regulation of protein ubiquitination
GO:0031625 ubiquitin protein ligase binding
GO:0042803 protein homodimerization activity
GO:0044419 interspecies interaction between organisms
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0047485 protein N-terminus binding
GO:0050681 androgen receptor binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of DAXX antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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