DAXX antibody | DAXX-01

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Mouse anti Human DAXX

Product Type
Monoclonal Antibody
Clone
DAXX-01
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2143 C IF WB 0.1 mg
Mouse anti Human DAXX antibody, clone DAXX-01 recognizes human Death domain-associated protein 6, also known as DAXX, ETS1-associated protein 1, EAP1 or Fas death domain-associated protein. DAXX is a 740 amino acid ~81 kDa nucleoplasmic protein.

DAXX is a regulator of apoptosis implicated in Fas and TGF beta induced apoptosis. DAXX is predominantly a nuclear protein, where it as a repressor of EtsI and Pax3 transactivation. When in association with ASK1 kinase DAXX translocates from the nucleus to the cytoplasm.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant C-terminal DAXX protein (aa 558-740).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9UER7 Related reagents
Entrez Gene
DAXX Related reagents
GO Terms
GO:0000775 chromosome, centromeric region
GO:0002039 p53 binding
GO:0006915 apoptosis
GO:0005057 receptor signaling protein activity
GO:0005730 nucleolus
GO:0006350 transcription
GO:0007257 activation of JUN kinase activity
GO:0008134 transcription factor binding
GO:0008625 induction of apoptosis via death domain receptors
GO:0016481 negative regulation of transcription
GO:0016605 PML body
GO:0031072 heat shock protein binding
GO:0030521 androgen receptor signaling pathway
GO:0031396 regulation of protein ubiquitination
GO:0031625 ubiquitin protein ligase binding
GO:0042803 protein homodimerization activity
GO:0044419 interspecies interaction between organisms
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0047485 protein N-terminus binding
GO:0050681 androgen receptor binding
Regulatory
For research purposes only

Applications of DAXX antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for DAXX antibody

  1. Boehrer, S. et al. (2005) Daxx overexpression in T-lymphoblastic Jurkat cellsenhances caspase-dependent death receptor- and drug-induced apoptosis in distinct ways.
    Cell Signal. 17:581-95.
  2. Tavalai, N. et al. (2006) Evidence for a role of the cellular ND10 protein PML in mediating intrinsic immunity against human cytomegalovirus infections.
    J Virol. 80: 8006-18.
  3. Sourvinos, G. et al. (2007) Recruitment of human cytomegalovirus immediate-early 2 protein onto parental viral genomes in association with ND10 in live-infected cells.
    J Virol. 81: 10123-36.
  4. Tavalai, N. et al. (2008) Insertion of an EYFP-pp71 (UL82) coding sequence into the human cytomegalovirus genome results in a recombinant virus with enhanced viral growth.
    J Virol. 82: 10543-55.
  5. Full, F. et al. (2012) Herpesvirus saimiri antagonizes nuclear domain 10-instituted intrinsic immunity via an ORF3-mediated selective degradation of cellular protein Sp100.
    J Virol. 86 (7): 3541-53.
  6. Full, F. et al. (2014) Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity.
    PLoS Pathog. 10(1):e1003863.
  7. Brown JR et al. (2016) The SUMO Ligase Protein Inhibitor of Activated STAT 1 (PIAS1) is a constituent PML-NB protein that contributes to the intrinsic antiviral immune response to herpes simplex virus 1 (HSV-1).
    J Virol. Apr 20. pii: JVI.00426-16. [Epub ahead of print]