Cytokeratin Type II antibody | AE3

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Mouse anti Human Cytokeratin Type II

Product Type
Monoclonal Antibody
Clone
AE3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA888H C P* 6 ml
Mouse anti Human cytokeratin type II antibody, clone AE3 recognizes human cytokeratin type II and reacts with most of the Basic (Type II) keratins. Including epidermal cell keratin of ~86 and 58 kDa and skin at ~58 kDa, 65 kDa and 67 kDa. Broad keratin reactivity is found on human epidermal cell, oesophagus and cornea.

Human epithelium contains at least one basic keratin, AE3 is broadly reactive and stains positively almost all epithelia and their neoplasms.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Chicken
Macaque
Bovine
Fish Expected from Sequence
Shrew
Rabbit
Mouse
Frog (Rana esculenta)
Rainbow Trout
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid, ready to use
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human epidermal cells.

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Cytokeratin Type II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen Neat
Immunohistology - Paraffin 1 Neat
  1. 1 This product requires protein digestion pre-treatment of paraffin sections. Antigen retrieval buffer pH8.0 (BUF025A) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human epidermal cell, Oesophagus, cornea.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Cytokeratin Type II antibody

  1. Woodcock-Mitchell, J. et al. (1982) Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
    J Cell Biol. 95 (2 Pt 1): 580-8.
  2. Merkel, V. et al. (2010) Distribution and phylogeny of immunoglobulin-binding protein G in Shiga toxin-producing Escherichia coli and its association with adherence phenotypes.
    Infect Immun. 78: 3625-36.
  3. Borrmann, E. et al. (2007) Campylobacter-induced interleukin-8 responses in human intestinal epithelial cells and primary intestinal chick cells.
    Vet Microbiol. 124: 115-24.
  4. Eichner, R. et al. (1984) Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression.
    J Cell Biol. 98 (4): 1388-96.
  5. Weiss, R.A. et al. (1984) Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes.
    J Cell Biol. 98 (4): 1397-406.
  6. Markl, J. & Franke, W.W. (1998) Localization of cytokeratins in tissues of the rainbow trout: Fundamental differences in expression pattern between fish and higher vertebrates.
    Differentiation 39: 97-122.
  7. Tsubura, A. et al. (1991) Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species.
    Acta Anat (Basel). 140 (3): 287-93.
  8. Fenoglio C et al. (2006) Morphofunctional evidence of changes in principal and mitochondria-rich cells in the epidermis of the frog Rana kl. esculenta living in a polluted habitat.
    Arch Environ Contam Toxicol. 51 (4): 690-702.