Cytokeratin Type II antibody | AE3

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Mouse anti Human Cytokeratin Type II

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
C P*
6 ml loader

Mouse anti Human cytokeratin type II antibody, clone AE3 recognizes human cytokeratin type II and reacts with most of the Basic (Type II) keratins. Including epidermal cell keratin of ~86 and 58 kDa and skin at ~58 kDa, 65 kDa and 67 kDa. Broad keratin reactivity is found on human epidermal cell, oesophagus and cornea.

Human epithelium contains at least one basic keratin, AE3 is broadly reactive and stains positively almost all epithelia and their neoplasms.

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Fish Expected from Sequence
Frog (Rana esculenta)
Rainbow Trout
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid, ready to use
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
1%Bovine Serum Albumin
Human epidermal cells.

Storage Information

Store at +4oC.


This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guaranteed until date of expiry. Please see product label.

More Information

For research purposes only

Applications of Cytokeratin Type II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen Neat
Immunohistology - Paraffin 1 Neat
  1. 1 This product requires protein digestion pre-treatment of paraffin sections. Antigen retrieval buffer pH8.0 (BUF025A) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human epidermal cell, Oesophagus, cornea.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

Source Reference

  1. Woodcock-Mitchell, J. et al. (1982) Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
    J Cell Biol. 95 (2 Pt 1): 580-8.

References for Cytokeratin Type II antibody

  1. Eichner, R. et al. (1984) Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression.
    J Cell Biol. 98 (4): 1388-96.
  2. Weiss, R.A. et al. (1984) Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes.
    J Cell Biol. 98 (4): 1397-406.
  3. Markl, J. & Franke, W.W. (1998) Localization of cytokeratins in tissues of the rainbow trout: Fundamental differences in expression pattern between fish and higher vertebrates.
    Differentiation 39: 97-122.
  4. Tsubura, A. et al. (1991) Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species.
    Acta Anat (Basel). 140 (3): 287-93.
  5. Fenoglio C et al. (2006) Morphofunctional evidence of changes in principal and mitochondria-rich cells in the epidermis of the frog Rana kl. esculenta living in a polluted habitat.
    Arch Environ Contam Toxicol. 51 (4): 690-702.
  6. Borrmann, E. et al. (2007) Campylobacter-induced interleukin-8 responses in human intestinal epithelial cells and primary intestinal chick cells.
    Vet Microbiol. 124: 115-24.
  7. Merkel, V. et al. (2010) Distribution and phylogeny of immunoglobulin-binding protein G in Shiga toxin-producing Escherichia coli and its association with adherence phenotypes.
    Infect Immun. 78: 3625-36.

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