Cytokeratin Type II antibody | AE3
Human epithelium contains at least one basic keratin, AE3 is broadly reactive and stains positively almost all epithelia and their neoplasms.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat Chicken Macaque Bovine Fish Expected from Sequence Shrew Rabbit Mouse Frog (Rana esculenta) Rainbow Trout
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid, ready to use
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide 1% Bovine Serum Albumin
- Human epidermal cells.
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed until date of expiry. Please see product label.
- For research purposes only
Applications of Cytokeratin Type II antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||Neat|
|Immunohistology - Paraffin 1||Neat|
- 1 This product requires protein digestion pre-treatment of paraffin sections. Antigen retrieval buffer pH8.0 (BUF025A) is recommended for this purpose.
- Histology Positive Control Tissue
- Human epidermal cell, Oesophagus, cornea.
Secondary Antibodies Available
Product Specific References
Woodcock-Mitchell, J. et al. (1982) Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
J Cell Biol. 95 (2 Pt 1): 580-8.
References for Cytokeratin Type II antibody
Eichner, R. et al. (1984) Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression.
J Cell Biol. 98 (4): 1388-96.
Weiss, R.A. et al. (1984) Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes.
J Cell Biol. 98 (4): 1397-406.
Markl, J. & Franke, W.W. (1998) Localization of cytokeratins in tissues of the rainbow trout: Fundamental differences in expression pattern between fish and higher vertebrates.
Differentiation 39: 97-122.
Tsubura, A. et al. (1991) Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species.
Acta Anat (Basel). 140 (3): 287-93.
Fenoglio C et al. (2006) Morphofunctional evidence of changes in principal and mitochondria-rich cells in the epidermis of the frog Rana kl. esculenta living in a polluted habitat.
Arch Environ Contam Toxicol. 51 (4): 690-702.
Borrmann, E. et al. (2007) Campylobacter-induced interleukin-8 responses in human intestinal epithelial cells and primary intestinal chick cells.
Vet Microbiol. 124: 115-24.
Merkel, V. et al. (2010) Distribution and phylogeny of immunoglobulin-binding protein G in Shiga toxin-producing Escherichia coli and its association with adherence phenotypes.
Infect Immun. 78: 3625-36.
How to Use the Spectraviewer?
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra