Cytokeratin antibody | AE1/AE3 Cocktail

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Anti Human Cytokeratin (Pan) Antibody, clone AE1/AE3 Cocktail gallery image 1 — Staining of human oesophagus. Formalin fixed, paraffin processed tissue with Mouse anti Human Cytokeratin clones AE1/AE3 (MCA1907T)

Mouse anti Human Cytokeratin (Pan)

Product Type: Monoclonal Antibody
Clone: AE1/AE3 Cocktail
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1907T	C P*			0.1 ml
MCA1907	C P*			1 ml

Mouse anti Human Cytokeratin (Pan) antibody, clone AE1/AE3 is a pan-cytokeratin reagent, being a cocktail of clones AE1 and AE3 which provides the broadest spectrum of reactivity to the 19 known human epidermal keratins. It produces positive staining in virtually all epithelia.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Pig
Macaque
Shrew

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

Pack Size: 0.1 ml

0.1% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Pack Size: 1 ml

0.1%	Sodium Azide
1%	Bovine Serum Albumin

Immunogen

Human epidermal keratins.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: Please see label for expiry date.

More Information

Regulatory: For research purposes only

Applications of Cytokeratin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen		1/10	1/40
Immunohistology - Paraffin ¹		1/10	1/40

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Serotec target unmasking fluid (STUF) product code BUF025A/B/C is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human Skin

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025B	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P

Product Specific References

References for Cytokeratin antibody

Smith, K.J. et al. (1998) Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin: including antibodies to proliferation antigens and cytokeratins with specificity in the skin.
J Dermatol Sci. 18 (1): 19-29.
Tsubura, A. et al. (1991) Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species.
Acta Anat (Basel). 140 (3): 287-93.

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