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Cytokeratin antibody | AE1/AE3 Cocktail

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Anti Human Cytokeratin (Pan) Antibody, clone AE1/AE3 Cocktail gallery image 1
Staining of human oesophagus. Formalin fixed, paraffin processed tissue with Mouse anti Human Cytokeratin clones AE1/AE3 (MCA1907T)

Mouse anti Human Cytokeratin (Pan)

Product Type
Monoclonal Antibody
Clone
AE1/AE3 Cocktail
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1907T
Datasheet
C P*
SDS
0.1 ml loader

MCA1907
Datasheet
C P*
SDS
1 ml loader

Mouse anti Human Cytokeratin (Pan) antibody, clone AE1/AE3 is a pan-cytokeratin reagent, being a cocktail of clones AE1 and AE3 which provides the broadest spectrum of reactivity to the 19 known human epidermal keratins. It produces positive staining in virtually all epithelia.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Pig
Macaque
Shrew
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 ml
0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Pack Size: 1 ml
0.1%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human epidermal keratins.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of Cytokeratin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/10 1/40
Immunohistology - Paraffin 1 1/10 1/40
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Serotec target unmasking fluid (STUF) product code BUF025A/B/C is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human Skin
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025B P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Product Specific References

References for Cytokeratin antibody

  1. Smith, K.J. et al. (1998) Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin: including antibodies to proliferation antigens and cytokeratins with specificity in the skin.
    J Dermatol Sci. 18 (1): 19-29.
  2. Tsubura, A. et al. (1991) Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species.
    Acta Anat (Basel). 140 (3): 287-93.

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