Cytokeratin antibody | AE1/AE3 Cocktail
Mouse anti Human Cytokeratin (Pan) antibody, clone AE1/AE3 is a pan-cytokeratin reagent, being a cocktail of clones AE1 and AE3 which provides the broadest spectrum of reactivity to the 19 known human epidermal keratins. It produces positive staining in virtually all epithelia.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Pig Macaque Shrew - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Pack Size: 1 ml
0.1% Sodium Azide 1% Bovine Serum Albumin Pack Size: 0.1 ml0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Human epidermal keratins.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- Regulatory
- For research purposes only
Applications of Cytokeratin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/10 | 1/40 | |
| Immunohistology - Paraffin 1 | 1/10 | 1/40 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Serotec target unmasking fluid (STUF) product code BUF025A/B/C is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human Skin
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 10x Antigen Retrieval Buffer, pH8.0 | BUF025B | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for Cytokeratin antibody
-
Smith, K.J. et al. (1998) Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin: including antibodies to proliferation antigens and cytokeratins with specificity in the skin.
J Dermatol Sci. 18 (1): 19-29. -
Tsubura, A. et al. (1991) Immunophenotypic difference of keratin expression in normal mammary glandular cells from five different species.
Acta Anat (Basel). 140 (3): 287-93.
Fluorescent Spectraviewer
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