Cytokeratin 8 antibody | UMAB1
Mouse anti Cytokeratin 8
Mouse anti human cytokeratin 8 antibody recognizes keratin, type II cytoskeletal 8, also known as KRT8, cytokeratin 8 or type-II keratin Kb8. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- UMAB1
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00402 | WB | 100 µl |
Mouse anti Human cytokeratin 8 antibody recognizes cytokeratin 8, also known as keratin 8 and keratin, type II cytoskeletal 8.
The KRT8 gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of KRT8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in KRT8 cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for KRT8 (provided by RefSeq, Jan 2012).
Mouse anti Human cytokeratin 8 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The KRT8 gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of KRT8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in KRT8 cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for KRT8 (provided by RefSeq, Jan 2012).
Mouse anti Human cytokeratin 8 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti cytokeratin 8 detects a band of approximately 55 kDa in MCF7 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein fragment corresponding to aa 91-381 of human cytokeratin 8 (NP_002264) produced in E.coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
More Information
- UniProt
- P05787 Related reagents
- Entrez Gene
- KRT8 Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Cytokeratin 8 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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