Cytokeratin 8 antibody | UMAB1

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Mouse anti Cytokeratin 8

Product Type
PrecisionAb Monoclonal
Clone
UMAB1
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00402
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human cytokeratin 8 antibody recognizes cytokeratin 8, also known as keratin 8 and keratin, type II cytoskeletal 8.

The KRT8 gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of KRT8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in KRT8 cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for KRT8 (provided by RefSeq, Jan 2012).

Mouse anti Human cytokeratin 8 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti cytokeratin 8 detects a band of approximately 55 kDa in MCF7 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 91-381 of human cytokeratin 8 (NP_002264) produced in E.coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P05787
Entrez Gene
KRT8
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Cytokeratin 8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml