Cytokeratin 7/17 antibody | C-46

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Mouse anti Human Cytokeratin 7/17

Product Type: Monoclonal Antibody
Clone: C-46
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2706	IP P* WB			0.1 mg

Mouse anti Human Cytokeratin 7/17 antibody, clone C-46 recognizes a unique epitope shared by the intermediate filament keratins, cytokeratin 7 and cytokeratin 17, a ~54 kDa type II basic cytokeratin, and ~46 kDa type I acidic cytokeratin respectively.

Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissues and their neoplasms, including the lungs, ovary and breast. Each keratin filament consists of at least one type I and one type II keratin chain, which then assemble to form a cytoskeletal network, extending from the surface of the nucleus to the cell membrane. The expression of a particular type of cytokeratin depends mainly on the type of epithelium and the development stage of the cell, including its corresponding malignant state. Immunohistochemical analysis of cytokeratin profiles is therefore a valuable tool for diagnosis and characterization, and this particular antibody could be useful for the evaluation of some stratified epithelia which express cytokeratin 17, but not cytokeratin 7.

Mouse anti Human Cytokeratin 7/17 antibody, clone C-46 is reported as suitable for use in immunocytochemistry (Bartek et al. 1991).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rabbit
Rat
Bovine
Pig

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Immunogen

HeLa cell cytoskeleton preparation.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: Q04695 Related reagents; P08729 Related reagents
Entrez Gene: KRT7 Related reagents; KRT17 Related reagents
GO Terms: GO:0006260 DNA replication; GO:0005515 protein binding; GO:0007010 cytoskeleton organization; GO:0005198 structural molecule activity; GO:0005634 nucleus; GO:0005794 Golgi apparatus; GO:0006417 regulation of translation; GO:0044419 interspecies interaction between organisms; GO:0045095 keratin filament; GO:0051325 interphase
Regulatory: For research purposes only

Applications of Cytokeratin 7/17 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunohistology - Paraffin ¹	20	30ug/ml
Immunoprecipitation
Western Blotting	2	3ug/ml

¹This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Pancreas.
Western Blotting: MCA2706 detects bands of approximately 54kDa and 46kDa in HeLa cytoskeleton cell lysates under reducing conditions.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Histar Detection System	STAR3000A	50 Tests	C P
Histar Detection System	STAR3000B	150 Tests	C P
Histar Detection System	STAR3000C	500 Tests	C P

Product Specific References

References for Cytokeratin 7/17 antibody

Bártek, J. et al. (1991) A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.
J Pathol. 164 (3): 215-24.
Vojtěsek, B. et al. (1990) A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.
Neoplasma. 37 (3): 333-42.

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