Cytokeratin 7/17 antibody | C-46

Mouse anti Human Cytokeratin 7/17

Product Type
Monoclonal Antibody
Clone
C-46
Isotype
IgG1
Specificity
Cytokeratin 7/17

Product Code Applications Pack Size List Price Your Price Qty
MCA2706
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP P * WB 0.1 mg loader
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loader
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Mouse anti Human Cytokeratin 7/17 antibody, clone C-46 recognizes a unique epitope shared by the intermediate filament keratins, cytokeratin 7 and cytokeratin 17, a ~54 kDa type II basic cytokeratin, and ~46 kDa type I acidic cytokeratin respectively.

Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissues and their neoplasms, including the lungs, ovary and breast. Each keratin filament consists of at least one type I and one type II keratin chain, which then assemble to form a cytoskeletal network, extending from the surface of the nucleus to the cell membrane. The expression of a particular type of cytokeratin depends mainly on the type of epithelium and the development stage of the cell, including its corresponding malignant state. Immunohistochemical analysis of cytokeratin profiles is therefore a valuable tool for diagnosis and characterization, and this particular antibody could be useful for the evaluation of some stratified epithelia which express cytokeratin 17, but not cytokeratin 7.

Mouse anti Human Cytokeratin 7/17 antibody, clone C-46 is reported as suitable for use in immunocytochemistry (Bartek et al. 1991).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rabbit
Rat
Bovine
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
HeLa cell cytoskeleton preparation.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 20 30ug/ml
Immunoprecipitation
Western Blotting 2 3ug/ml
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Pancreas.
Western Blotting
MCA2706 detects bands of approximately 54kDa and 46kDa in HeLa cytoskeleton cell lysates under reducing conditions.

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References for Cytokeratin 7/17 antibody

  1. Bártek, J. et al. (1991) A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.
    J Pathol. 164 (3): 215-24.
  2. Vojtěsek, B. et al. (1990) A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.
    Neoplasma. 37 (3): 333-42.

RRID
AB_2234503
UniProt
Q04695
P08729
Entrez Gene
KRT7
KRT17
GO Terms
GO:0006260 DNA replication
GO:0005515 protein binding
GO:0007010 cytoskeleton organization
GO:0005198 structural molecule activity
GO:0005634 nucleus
GO:0005794 Golgi apparatus
GO:0006417 regulation of translation
GO:0044419 interspecies interaction between organisms
GO:0045095 keratin filament
GO:0051325 interphase
View more products with CYTOKERATIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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