Cytokeratin 7/17 antibody | C-46
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Mouse anti Human Cytokeratin 7/17 antibody, clone C-46 recognizes a unique epitope shared by the intermediate filament keratins, cytokeratin 7 and cytokeratin 17, a ~54 kDa type II basic cytokeratin, and ~46 kDa type I acidic cytokeratin respectively.
Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissues and their neoplasms, including the lungs, ovary and breast. Each keratin filament consists of at least one type I and one type II keratin chain, which then assemble to form a cytoskeletal network, extending from the surface of the nucleus to the cell membrane. The expression of a particular type of cytokeratin depends mainly on the type of epithelium and the development stage of the cell, including its corresponding malignant state. Immunohistochemical analysis of cytokeratin profiles is therefore a valuable tool for diagnosis and characterization, and this particular antibody could be useful for the evaluation of some stratified epithelia which express cytokeratin 17, but not cytokeratin 7.
Mouse anti Human Cytokeratin 7/17 antibody, clone C-46 is reported as suitable for use in immunocytochemistry (Bartek et al. 1991).
Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissues and their neoplasms, including the lungs, ovary and breast. Each keratin filament consists of at least one type I and one type II keratin chain, which then assemble to form a cytoskeletal network, extending from the surface of the nucleus to the cell membrane. The expression of a particular type of cytokeratin depends mainly on the type of epithelium and the development stage of the cell, including its corresponding malignant state. Immunohistochemical analysis of cytokeratin profiles is therefore a valuable tool for diagnosis and characterization, and this particular antibody could be useful for the evaluation of some stratified epithelia which express cytokeratin 17, but not cytokeratin 7.
Mouse anti Human Cytokeratin 7/17 antibody, clone C-46 is reported as suitable for use in immunocytochemistry (Bartek et al. 1991).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rabbit Rat Bovine Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- HeLa cell cytoskeleton preparation.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q04695
- P08729
- Entrez Gene
- KRT7
- KRT17
- GO Terms
- GO:0006260 DNA replication
- GO:0005515 protein binding
- GO:0007010 cytoskeleton organization
- GO:0005198 structural molecule activity
- GO:0005634 nucleus
- GO:0005794 Golgi apparatus
- GO:0006417 regulation of translation
- GO:0044419 interspecies interaction between organisms
- GO:0045095 keratin filament
- GO:0051325 interphase
- Regulatory
- For research purposes only
Applications of Cytokeratin 7/17 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 20 | 30ug/ml | |
| Immunoprecipitation | |||
| Western Blotting | 2 | 3ug/ml |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Pancreas.
- Western Blotting
- MCA2706 detects bands of approximately 54kDa and 46kDa in HeLa cytoskeleton cell lysates under reducing conditions.
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Histar Detection System | STAR3000A | C P | 50 Tests |
Product Specific References
References for Cytokeratin 7/17 antibody
-
Bártek, J. et al. (1991) A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.
J Pathol. 164 (3): 215-24. -
Vojtěsek, B. et al. (1990) A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.
Neoplasma. 37 (3): 333-42.
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