Cytokeratin 7/17 antibody | C-46

Mouse anti Human Cytokeratin 7/17 antibody, clone C-46 recognizes a unique epitope shared by the intermediate filament keratins, cytokeratin 7 and cytokeratin 17, a ~54 kDa type II basic cytokeratin, and ~46 kDa type I acidic cytokeratin respectively.

Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissues and their neoplasms, including the lungs, ovary and breast. Each keratin filament consists of at least one type I and one type II keratin chain, which then assemble to form a cytoskeletal network, extending from the surface of the nucleus to the cell membrane. The expression of a particular type of cytokeratin depends mainly on the type of epithelium and the development stage of the cell, including its corresponding malignant state. Immunohistochemical analysis of cytokeratin profiles is therefore a valuable tool for diagnosis and characterization, and this particular antibody could be useful for the evaluation of some stratified epithelia which express cytokeratin 17, but not cytokeratin 7.

Mouse anti Human Cytokeratin 7/17 antibody, clone C-46 is reported as suitable for use in immunocytochemistry (Bartek et al. 1991).

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin 1		20	30ug/ml
Immunoprecipitation
Western Blotting		2	3ug/ml

1. ¹This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

Histology Positive Control Tissue

Pancreas.

Western Blotting

MCA2706 detects bands of approximately 54kDa and 46kDa in HeLa cytoskeleton cell lysates under reducing conditions.