Cytokeratin 5 antibody

Mouse anti Cytokeratin 5

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
Cytokeratin 5

Product Code Applications Pack Size List Price Your Price Qty
VMA00119
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human cytokeratin 5 antibody recognizes cytokeratin 5, also known as 58 kDa cytokeratin, CK-5, cytokeratin-5, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types), keratin, type II cytoskeletal 5 and type-II keratin Kb5.

Encoded by KRT5, cytokeratin 5 is a member of the keratin protein family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13 (provided by RefSeq, Jul 2008).

Mouse anti Human cytokeratin 5 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti cytokeratin 5 detects a band of approximately 62 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified recombinant fragment of human cytokeratin 5 expressed in E. coli
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P13647
Entrez Gene
KRT5
GO Terms
GO:0005515 protein binding
GO:0005829 cytosol
GO:0005200 structural constituent of cytoskeleton
GO:0008544 epidermis development
GO:0031581 hemidesmosome assembly
GO:0045095 keratin filament

VMA00119

120615

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