Cytokeratin 5 antibody | OTI1C7
Mouse anti Cytokeratin 5
Cytokeratin 5 antibody recognizes keratin, type II cytoskeletal 5, also known as 58 kDa cytokeratin, CK-5, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types or type-II keratin Kb5. This antibody has been extensively validated for western blotting using whole cell lysates.
Cytokeratin 5 Antibody with Control Lysate
Cytokeratin 5 antibody recognizes keratin, type II cytoskeletal 5, also known as 58 kDa cytokeratin, CK-5, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types or type-II keratin Kb5. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI1C7
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00267 | WB | 100 µl | ||||
| VMA00267KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human cytokeratin 5 antibody recognizes the keratin, type II cytoskeletal 5, also known as 58 kDa cytokeratin, CK-5, cytokeratin-5, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types), keratin, type II cytoskeletal 5 and type-II keratin Kb5.
The protein encoded by KRT5 gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13 (provided by RefSeq, Jul 2008).
Mouse anti Human cytokeratin 5 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by KRT5 gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13 (provided by RefSeq, Jul 2008).
Mouse anti Human cytokeratin 5 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti cytokeratin 5 detects a band of approximately 62 kDa in A431 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Synthetic peptide (EQGTKTVRQN LEPLFEQYIN NLRRQ) corresponding to a conserved region of human cytokeratin 5
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg A431 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P13647 Related reagents
- Entrez Gene
- KRT5 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0008544 epidermis development
- GO:0031581 hemidesmosome assembly
- GO:0045095 keratin filament
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Cytokeratin 5 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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