Cytokeratin 20 antibody
Human anti Cytokeratin 20
Anti Human cytokeratin 20 antibody recognizes keratin, type I cytoskeletal 20, also known as KRT20, K20, CD20, cytokeratin-20, CK20 or keratin-20. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- HuCAL Fab bivalent
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00451 | WB | 100 µl |
Human anti Human cytokeratin 20 antibody recognizes cytokeratin 20, also known as KRT20, keratin, type I cytoskeletal 20 and keratin-20, protein IT.
The protein encoded by the KRT20 gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21 (provided by RefSeq, Jul 2008).
Human anti Human cytokeratin 20 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the KRT20 gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21 (provided by RefSeq, Jul 2008).
Human anti Human cytokeratin 20 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti cytokeratin 20 detects a band of approximately 44 kDa in MCF7 cell lysates
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human cytokeratin 20
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P35900 Related reagents
- Entrez Gene
- KRT20 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0005882 intermediate filament
- GO:0006915 apoptosis
- GO:0005200 structural constituent of cytoskeleton
- GO:0005794 Golgi apparatus
- GO:0045109 intermediate filament organization
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
- Regulatory
- For research purposes only
Applications of Cytokeratin 20 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | STAR126P | 0.5 ml | E WB |
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