Cytokeratin 20 antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti Cytokeratin 20 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with cytokeratin 20 antibody followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to cytokeratin 20 (molecular weight 44 kDa)

Human anti Cytokeratin 20

Product Type: PrecisionAb Monoclonal
Isotype: HuCAL Fab bivalent
Format: Purified
Specificity: Cytokeratin 20

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00451 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
	Log in

Human anti Human cytokeratin 20 antibody recognizes cytokeratin 20, also known as KRT20, keratin, type I cytoskeletal 20 and keratin-20, protein IT.

The protein encoded by the KRT20 gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21 (provided by RefSeq, Jul 2008).

Human anti Human cytokeratin 20 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti cytokeratin 20 detects a band of approximately 44 kDa in MCF7 cell lysates.
Product Form: A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation: Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant human cytokeratin 20
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Human IgG F(ab')2:HRP	STAR126P	E WB	0.5 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Human IgG F(ab')2:HRP

UniProt: P35900
Entrez Gene: KRT20
GO Terms: GO:0005515 protein binding; GO:0005882 intermediate filament; GO:0006915 apoptosis; GO:0005200 structural constituent of cytoskeleton; GO:0005794 Golgi apparatus; GO:0045109 intermediate filament organization