Cytokeratin 20 antibody

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Human anti Cytokeratin 20

Anti Human cytokeratin 20 antibody recognizes keratin, type I cytoskeletal 20, also known as KRT20, K20, CD20, cytokeratin-20, CK20 or keratin-20. This antibody has been extensively validated for western blotting using whole cell lysates.

Cytokeratin 20 Antibody with Control Lysate

Anti Human cytokeratin 20 antibody recognizes keratin, type I cytoskeletal 20, also known as KRT20, K20, CD20, cytokeratin-20, CK20 or keratin-20. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00451 WB 100 µl
VMA00451KT WB 2 Westerns
With Lysate 400µg
Human anti Human cytokeratin 20 antibody recognizes cytokeratin 20, also known as KRT20, keratin, type I cytoskeletal 20 and keratin-20, protein IT.

The protein encoded by the KRT20 gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21 (provided by RefSeq, Jul 2008).

Human anti Human cytokeratin 20 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Product Form
Purified IgG - liquid
Preparation
Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
Preparation
20μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human cytokeratin 20

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P35900 Related reagents
Entrez Gene
KRT20 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005882 intermediate filament
GO:0006915 apoptosis
GO:0005200 structural constituent of cytoskeleton
GO:0005794 Golgi apparatus
GO:0045109 intermediate filament organization
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Cytokeratin 20 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti cytokeratin 20 detects a band of approximately 44 kDa in MCF7 cell lysates
Western Blotting
Anti cytokeratin 20 detects a band of approximately 44 kDa in MCF7 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB