Cytokeratin 19 antibody | BA17
Mouse anti Human Cytokeratin 19 antibody, clone BA17 recognizes the ~40 kDa cytokeratin corresponding to cytokeratin 19.
Cytokeratin 19 is expressed by many simple and non-keratinizing normal and neoplastic epithelia, including glandular and ductal epithelia.
Cytokeratin 19 is expressed by many simple and non-keratinizing normal and neoplastic epithelia, including glandular and ductal epithelia.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Mammary organoids.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of Cytokeratin 19 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | 1/50 | 1/100 | |
| Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. Antigen retrieval can also be performed by protein digestion e.g. trypsin or pronase
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Normal breast
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Secondary Antibodies Available
Product Specific References
References for Cytokeratin 19 antibody
-
Bartek, J. et al. (1985) A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins.
J Cell Sci. 75: 17-33. -
Bartek, J. et al. (1985) Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours.
Int J Cancer. 36 (3): 299-306. -
Chen, Y.K. et al. (2006) Ductular proliferation in liver tissues with severe chronic hepatitis B: an immunohistochemical study.
World J Gastroenterol. 12: 1443-6.
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