Cytokeratin 19 antibody | BA17

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Anti Human Cytokeratin 19 Antibody, clone BA17 thumbnail image 1

Anti Human Cytokeratin 19 Antibody, clone BA17 thumbnail image 2

Anti Human Cytokeratin 19 Antibody, clone BA17 thumbnail image 3

Anti Human Cytokeratin 19 Antibody, clone BA17 gallery image 1 — Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 19 antibody (**MCA2145**) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power).

Anti Human Cytokeratin 19 Antibody, clone BA17 gallery image 2 — Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 19 antibody (**MCA2145**) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power).

Anti Human Cytokeratin 19 Antibody, clone BA17 gallery image 3 — Sandwich ELISA analysis of human cytokeratin-19 expression using Mouse anti Human cytokeratin 19 antibody, clone BA19 (**MCA2145**) as a capture reagent and biotinylated Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2 (5552-9009) as a detection reagent with purified human cytokeratin 19 (Bio-rad catalog; 34002301) as antigen to create the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green), colorectal cancer serum (orange) and breast cancer serum (red) samples at 1:4 dilution are shown.

Mouse anti Human Cytokeratin 19

Product Type: Monoclonal Antibody
Clone: BA17
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2145	C P* WB			0.2 mg

Mouse anti Human Cytokeratin 19 antibody, clone BA17 recognizes the ~40 kDa cytokeratin corresponding to cytokeratin 19.

Cytokeratin 19 is expressed by many simple and non-keratinizing normal and neoplastic epithelia, including glandular and ductal epithelia.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Mammary organoids.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P08727 Related reagents
Entrez Gene: KRT19 Related reagents
Regulatory: For research purposes only

Applications of Cytokeratin 19 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin ¹	1/50	1/100
Western Blotting

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. Antigen retrieval can also be performed by protein digestion e.g. trypsin or pronase

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Normal breast

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Cytokeratin 19 antibody

Bartek, J. et al. (1985) A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins.
J Cell Sci. 75: 17-33.
Bartek, J. et al. (1985) Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours.
Int J Cancer. 36 (3): 299-306.
Chen, Y.K. et al. (2006) Ductular proliferation in liver tissues with severe chronic hepatitis B: an immunohistochemical study.
World J Gastroenterol. 12: 1443-6.

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