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Cytokeratin 19 antibody | BA17

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Anti Human Cytokeratin 19 Antibody, clone BA17 thumbnail image 1 Anti Human Cytokeratin 19 Antibody, clone BA17 thumbnail image 2 Anti Human Cytokeratin 19 Antibody, clone BA17 thumbnail image 3
Anti Human Cytokeratin 19 Antibody, clone BA17 gallery image 1
Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 19 antibody (MCA2145) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power).
Anti Human Cytokeratin 19 Antibody, clone BA17 gallery image 2
Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 19 antibody (MCA2145) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power).
Anti Human Cytokeratin 19 Antibody, clone BA17 gallery image 3
Sandwich ELISA analysis of human cytokeratin-19 expression using Mouse anti Human cytokeratin 19 antibody, clone BA19 (MCA2145) as a capture reagent and biotinylated Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2 (5552-9009) as a detection reagent with purified human cytokeratin 19 (Bio-rad catalog; 34002301) as antigen to create the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green), colorectal cancer serum (orange) and breast cancer serum (red) samples at 1:4 dilution are shown.

Mouse anti Human Cytokeratin 19

Product Type
Monoclonal Antibody
Clone
BA17
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2145
Datasheet
C P* WB
MSDS
0.2 mg loader

Mouse anti Human Cytokeratin 19 antibody, clone BA17 recognizes the ~40 kDa cytokeratin corresponding to cytokeratin 19.

Cytokeratin 19 is expressed by many simple and non-keratinizing normal and neoplastic epithelia, including glandular and ductal epithelia.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Mammary organoids.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P08727
Entrez Gene
KRT19
Regulatory
For research purposes only

Applications of Cytokeratin 19 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/50 1/100
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. Antigen retrieval can also be performed by protein digestion e.g. trypsin or pronase
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Normal breast
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Cytokeratin 19 antibody

  1. Bartek, J. et al. (1985) A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins.
    J Cell Sci. 75: 17-33.
  2. Bartek, J. et al. (1985) Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours.
    Int J Cancer. 36 (3): 299-306.
  3. Chen, Y.K. et al. (2006) Ductular proliferation in liver tissues with severe chronic hepatitis B: an immunohistochemical study.
    World J Gastroenterol. 12: 1443-6.
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