Cytokeratin 19/20 antibody

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Anti Cytokeratin 19/20 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with cytokeratin 19/20 antibody (**VMA00009**) followed by detection with HRP conjugated Goat anti Human IgG F(ab')₂ (1/2500, STAR126P) and visualized on the ChemiDoc MP with 5 second exposure. Arrows point to cytokeratin 19/20 (molecular weight 40, 45 kDa).

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Human anti Cytokeratin 19/20

Product Type: PrecisionAb Monoclonal
Isotype: HuCAL Fab bivalent
Format: Purified
Specificity: Cytokeratin 19/20

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00009 Datasheet Datasheet SDS SDS	E WB	100 µl		Log in	Get Quote
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Human anti Human cytokeratin 19/20 antibody recognizes human cytokeratin 20, and has been demonstrated to cross-react with cytokeratin 19. In immunohistology reactivity is seen both with tissues expressing only CK19 and with those expressing only CK20.

Human anti Human cytokeratin 19/20 antibody recognizes cytokeratins 19 and 20 as bands of ~40 kDa and ~45 kDa respectively by western blotting under reducing conditions.

Target Species: Human
Western Blotting: Anti cytokeratin 19/20 antibody detects bands of approximately 40 kDa and 45 kDa in HeLa cell lysates.
Product Form: A bivalent human recombinant Fab selected from the HuCAL GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation: Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Source: E.coli
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant human cytokeratin 20
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt: P35900; P08727
Entrez Gene: KRT19; KRT20
GO Terms: GO:0005515 protein binding; GO:0005882 intermediate filament; GO:0006915 apoptosis; GO:0005200 structural constituent of cytoskeleton; GO:0005794 Golgi apparatus; GO:0045109 intermediate filament organization