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Cytokeratin 19/20 antibody

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Western Blot Validated
Anti Cytokeratin 19/20 Antibody (PrecisionAb™ Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with cytokeratin 19/20 antibody (VMA00009) followed by detection with HRP conjugated Goat anti Human IgG F(ab')2 (1/2500, STAR126P) and visualized on the ChemiDoc MP with 5 second exposure. Arrows point to cytokeratin 19/20 (molecular weight 40, 45 kDa).

Human anti Cytokeratin 19/20

Cytokeratin 19/20 antibody recognizes human cytokeratin 20, and cross reacts with cytokeratin 19, also known as type I cytoskeletal 19 or keratin-19. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
PrecisionAb Antibodies
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00009 WB 100 µl
Human anti Human cytokeratin 19/20 antibody recognizes human cytokeratin 20, and has been demonstrated to cross-react with cytokeratin 19. In immunohistology reactivity is seen both with tissues expressing only CK19 and with those expressing only CK20.

Human anti Human cytokeratin 19/20 antibody recognizes cytokeratins 19 and 20 as bands of ~40 kDa and ~45 kDa respectively by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti cytokeratin 19/20 antibody detects bands of approximately 40 kDa and 45 kDa in HeLa cell lysates.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation
Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human cytokeratin 20
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P35900 Related reagents
P08727 Related reagents
Entrez Gene
KRT19 Related reagents
KRT20 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005882 intermediate filament
GO:0006915 apoptosis
GO:0005200 structural constituent of cytoskeleton
GO:0005794 Golgi apparatus
GO:0045109 intermediate filament organization
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of Cytokeratin 19/20 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB
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