Cytokeratin 18 antibody

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Human anti Cytokeratin 18

Anti human Cytokeratin 18 antibody recognizes keratin, type I cytoskeletal 18, also known as K18, CK-18, cell proliferation-inducing gene 46 protein, cytokeratin 18 or keratin-18. This antibody has been extensively validated for western blotting using whole cell lysates.

Cytokeratin 18 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Isotype: HuCAL Fab bivalent
Format: Purified

Product Code	Applications	Pack Size
VMA00444	WB	100 µl
VMA00444KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Human anti Human cytokeratin 18 antibody recognizes cytokeratin 18, also known as K18, CK-18, cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, keratin type I cytoskeletal 18 or simply keratin-18.

Encoded by the KRT18 gene, cytokeratin 18 together with its filament partner cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in KRT18 have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for KRT18 (provided by RefSeq, Jul 2008).

Human anti Human cytokeratin 18 antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti cytokeratin 18 detects a band of approximately 46 kDa in MCF7 cell lysates
Product Form: A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase (BAP) fusion protein. The BAP enzymatic activity is silenced by mutation. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Product Form: Purified IgG - liquid
Preparation: Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
Preparation: 20μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Cytokeratin 18
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg MCF7 lysate lyophilized in RIPA buffer
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: P05783 Related reagents
Entrez Gene: KRT18 Related reagents
GO Terms: GO:0005515 protein binding; GO:0005198 structural molecule activity; GO:0007049 cell cycle; GO:0009653 anatomical structure morphogenesis; GO:0034451 centriolar satellite; GO:0043000 Golgi to plasma membrane CFTR protein transport; GO:0043066 negative regulation of apoptosis; GO:0044419 interspecies interaction between organisms; GO:0045095 keratin filament; GO:0048471 perinuclear region of cytoplasm
Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of Cytokeratin 18 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Human IgG F(ab')2:HRP	STAR126P	0.5 ml	E WB
Goat anti Human IgG F(ab')2:HRP	STAR126P	0.5 ml	E WB

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