Cytokeratin 18 antibody

100% Secure


Human anti Cytokeratin 18

Anti human Cytokeratin 18 antibody recognizes keratin, type I cytoskeletal 18, also known as K18, CK-18, cell proliferation-inducing gene 46 protein, cytokeratin 18 or keratin-18. This antibody has been extensively validated for western blotting using whole cell lysates.

Cytokeratin 18 Antibody with Control Lysate

Anti human Cytokeratin 18 antibody recognizes keratin, type I cytoskeletal 18, also known as K18, CK-18, cell proliferation-inducing gene 46 protein, cytokeratin 18 or keratin-18. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00444 WB 100 µl
VMA00444KT WB 2 Westerns
With Lysate 400µg
Human anti Human cytokeratin 18 antibody recognizes cytokeratin 18, also known as K18, CK-18, cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, keratin type I cytoskeletal 18 or simply keratin-18.

Encoded by the KRT18 gene, cytokeratin 18 together with its filament partner cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in KRT18 have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for KRT18 (provided by RefSeq, Jul 2008).

Human anti Human cytokeratin 18 antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase (BAP) fusion protein. The BAP enzymatic activity is silenced by mutation. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Product Form
Purified IgG - liquid
Preparation
Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
Preparation
20μl Human recombinant monoclonal antibody purified by StrepTactin affinity chromatography from E. coli extracts
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Cytokeratin 18

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P05783 Related reagents
Entrez Gene
KRT18 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005198 structural molecule activity
GO:0007049 cell cycle
GO:0009653 anatomical structure morphogenesis
GO:0034451 centriolar satellite
GO:0043000 Golgi to plasma membrane CFTR protein transport
GO:0043066 negative regulation of apoptosis
GO:0044419 interspecies interaction between organisms
GO:0045095 keratin filament
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Cytokeratin 18 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti cytokeratin 18 detects a band of approximately 46 kDa in MCF7 cell lysates
Western Blotting
Anti cytokeratin 18 detects a band of approximately 46 kDa in MCF7 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB