Cytokeratin 18 antibody

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Anti Cytokeratin 18 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with cytokeratin 18 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to cytokeratin 18 (molecular weight 46 kDa)

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Mouse anti Cytokeratin 18

Product Type: PrecisionAb Monoclonal
Isotype: IgG2b
Format: Purified
Specificity: Cytokeratin 18

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VMA00314 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human cytokeratin 18 antibody recognizes cytokeratin-18, also known as keratin, type I cytoskeletal 18, CK-18, cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, keratin, type I cytoskeletal 18 and keratin-18.

Encoded by the KRT18 gene cytokeratin 18, together with its filament partner cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in KRT18 have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for KRT18 (provided by RefSeq, Jul 2008).

Mouse anti Human cytokeratin 18 antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti cytokeratin 18 detects a band of approximately 46 kDa in MCF7 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant protein corresponding to amino acid 391-483 of human cytokeratin 18 expressed in E. coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P05783
Entrez Gene: KRT18
GO Terms: GO:0005515 protein binding; GO:0005198 structural molecule activity; GO:0007049 cell cycle; GO:0009653 anatomical structure morphogenesis; GO:0034451 centriolar satellite; GO:0043000 Golgi to plasma membrane CFTR protein transport; GO:0043066 negative regulation of apoptosis; GO:0044419 interspecies interaction between organisms; GO:0045095 keratin filament; View More GO Terms; GO:0048471 perinuclear region of cytoplasm