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Cytokeratin 18 antibody

Mouse anti Cytokeratin 18

Product Type
PrecisionAb Monoclonal
Isotype
IgG2b
Format
Purified
Specificity
Cytokeratin 18

Product Code Applications Pack Size List Price Your Price Qty
VMA00314
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human cytokeratin 18 antibody recognizes cytokeratin-18, also known as keratin, type I cytoskeletal 18, CK-18, cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, keratin, type I cytoskeletal 18 and keratin-18.

Encoded by the KRT18 gene cytokeratin 18, together with its filament partner cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in KRT18 have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for KRT18 (provided by RefSeq, Jul 2008).

Mouse anti Human cytokeratin 18 antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti cytokeratin 18 detects a band of approximately 46 kDa in MCF7 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein corresponding to amino acid 391-483 of human cytokeratin 18 expressed in E. coli
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P05783
Entrez Gene
KRT18
GO Terms
GO:0005515 protein binding
GO:0005198 structural molecule activity
GO:0007049 cell cycle
GO:0009653 anatomical structure morphogenesis
GO:0034451 centriolar satellite
GO:0043000 Golgi to plasma membrane CFTR protein transport
GO:0043066 negative regulation of apoptosis
GO:0044419 interspecies interaction between organisms
GO:0045095 keratin filament
GO:0048471 perinuclear region of cytoplasm

VMA00314

151112

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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