Cytokeratin 18 antibody | CY90

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Mouse anti Human Cytokeratin 18

Product Type
Monoclonal Antibody
Clone
CY90
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1864HT C IF P* 1 ml
MCA1864H C IF P* 5 ml
Mouse anti Human Cytokeratin 18 antibody, clone CY90 recognizes human cytokeratin 18, a 45 kDa member of the acidic family of Cytokeratins. Cytokeratin 18 is often expressed in combination with cytokeratin 8. This antibody stains a range of simple epithelial, but not stratified squamous epithelia.

Product Details

Target Species
Human
Product Form
Ready To Use
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Human A-431 and MCF-7 cell lines

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P05783 Related reagents
Entrez Gene
KRT18 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005198 structural molecule activity
GO:0007049 cell cycle
GO:0009653 anatomical structure morphogenesis
GO:0034451 centriolar satellite
GO:0043000 Golgi to plasma membrane CFTR protein transport
GO:0043066 negative regulation of apoptosis
GO:0044419 interspecies interaction between organisms
GO:0045095 keratin filament
GO:0048471 perinuclear region of cytoplasm
Regulatory
For research purposes only

Applications of Cytokeratin 18 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen Neat
Immunohistology - Paraffin 1 Neat
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Serotec target unmasking fluid (STUF) product code BUF025A is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue
Intestine

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Cytokeratin 18 antibody

  1. MacFarlane, M. et al. (2000) Active caspases and cleaved cytokeratins are sequestered into cytoplasmic inclusions in TRAIL-induced apoptosis.
    J Cell Biol. 148 (6): 1239-54.
  2. Holliday, D. et al. (2009) Novel multicellular organotypic models of normal and malignant breast: tools for dissecting the role of the microenvironment in breast cancer progression.
    Breast Cancer Res 11: R3
  3. Clark, S.E. et al. (2011) Molecular subtyping of DCIS: heterogeneity of breast cancer reflected in pre-invasive disease.
    Br J Cancer. 2011 Jan 4;104(1):120-7
  4. Ginzberg, H.H. et al (2001) Neutrophil-mediated epithelial injury during transmigration: role of elastase.
    Am J Physiol Gastrointest Liver Physiol. 281: G705-17.