Cytokeratin 18/19 antibody
Human anti Cytokeratin 18/19
Cytokeratin 18/19 antibody recognizes the keratin, type I cytoskeletal 18 , also known as cell proliferation-inducing gene 46, protein cytokeratin-18, CK-18, keratin-18 or K18. The antibody has been extensively validated for western blotting using whole cell lysates.
Cytokeratin 18/19 Antibody with Control Lysate
Cytokeratin 18/19 antibody recognizes the keratin, type I cytoskeletal 18 , also known as cell proliferation-inducing gene 46, protein cytokeratin-18, CK-18, keratin-18 or K18. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- HuCAL Fab bivalent
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00011 | WB | 100 µl | ||||
| VMA00011KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Human anti Human cytokeratin 18/19 antibody recognizes human cytokeratin 18, a amino acid type I intermediate filament protein expressed by epithelial cells. The intermediate filaments are expressed as heterodimers in epithelial tissues. Cytokeratin 18 (CK18) is often co-expressed with cytokeratin 8 (CK8). The CK18/CK8 heterodimer is the major cytokeratin pair found in simple epithelia such as that found in the liver, intestine and breast ducts. CK18 plays an important role in maintaining epithelial cell integrity.
This clone has also been demonstrated to cross-react with cytokeratin 19. Human anti Human cytokeratin 18/19 antibody recognizes cytokeratin 18 as a band of ~48 kDa and cytokeratin 19 as a band of ~44 kDa in western blotting under reducing conditions.
This clone has also been demonstrated to cross-react with cytokeratin 19. Human anti Human cytokeratin 18/19 antibody recognizes cytokeratin 18 as a band of ~48 kDa and cytokeratin 19 as a band of ~44 kDa in western blotting under reducing conditions.
Product Details
- Target Species
- Human
- Western Blotting
- Anti cytokeratin 18/19 antibody detects bands of approximately 44 kDa in HeLa cell lysates.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
- Preparation
- Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
- Preparation
- 20μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human cytokeratin 18
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P05783 Related reagents
- P08727 Related reagents
- Entrez Gene
- KRT18 Related reagents
- KRT19 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0005198 structural molecule activity
- GO:0007049 cell cycle
- GO:0009653 anatomical structure morphogenesis
- GO:0034451 centriolar satellite
- GO:0043000 Golgi to plasma membrane CFTR protein transport
- GO:0043066 negative regulation of apoptosis
- GO:0044419 interspecies interaction between organisms
- GO:0045095 keratin filament
- GO:0048471 perinuclear region of cytoplasm
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
- Regulatory
- For research purposes only
Applications of Cytokeratin 18/19 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
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| Mouse anti Penta Histidine Tag:HRP | MCA5995P | 125 µl | E WB | ||
| Goat anti Human IgG F(ab')2:HRP | STAR126P | 0.5 ml | E WB | ||
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| Goat anti Human IgG F(ab')2:HRP | STAR126P | 0.5 ml | E WB |
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