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Cytokeratin 18/19 antibody

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Western Blot Validated
Anti Cytokeratin 18/19 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with cytokeratin 18/19 antibody (VMA00011) followed by detection with HRP conjugated Goat anti Human IgG F(ab')2 (1/2500, STAR126P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to cytokeratin 18/19 (molecular weight 48, 44 kDa).

Human anti Cytokeratin 18/19

Product Type
PrecisionAb Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00011
Datasheet
WB
SDS
100 µl loader

Human anti Human cytokeratin 18/19 antibody recognizes human cytokeratin 18, a amino acid type I intermediate filament protein expressed by epithelial cells. The intermediate filaments are expressed as heterodimers in epithelial tissues. Cytokeratin 18 (CK18) is often co-expressed with cytokeratin 8 (CK8). The CK18/CK8 heterodimer is the major cytokeratin pair found in simple epithelia such as that found in the liver, intestine and breast ducts. CK18 plays an important role in maintaining epithelial cell integrity.

This clone has also been demonstrated to cross-react with cytokeratin 19. Human anti Human cytokeratin 18/19 antibody recognizes cytokeratin 18 as a band of ~48 kDa and cytokeratin 19 as a band of ~44 kDa in western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti cytokeratin 18/19 antibody detects bands of approximately 44 kDa in HeLa cell lysates.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation
Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human cytokeratin 18

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P05783
P08727
Entrez Gene
KRT18
KRT19
GO Terms
GO:0005515 protein binding
GO:0005198 structural molecule activity
GO:0007049 cell cycle
GO:0009653 anatomical structure morphogenesis
GO:0034451 centriolar satellite
GO:0043000 Golgi to plasma membrane CFTR protein transport
GO:0043066 negative regulation of apoptosis
GO:0044419 interspecies interaction between organisms
GO:0045095 keratin filament
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of Cytokeratin 18/19 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader

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