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Cytokeratin 18/19 antibody | AbD03748

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Anti Human Cytokeratin 18/19 Antibody, clone AbD03748 gallery image 1
MCF-7 human breast adenocarcinoma cell lysate probed with Human anti Human cytokeratin 18/19 (HCA077).

Human anti Human Cytokeratin 18/19

Product Type
Monoclonal Antibody
Clone
AbD03748
Isotype
HuCAL Fab bivalent
Product Code Applications Pack Size List Price Quantity
HCA077
Datasheet
E P* WB
MSDS
0.1 mg loader

Human anti Human cytokeratin 18/19 antibody, clone AbD03748 recognizes human cytokeratin 18 (CK18), a 45 kDa type I intermediate filament protein expressed by epithelial cells. The intermediate filaments are expressed as heterodimers in epithelial tissues. CK18 is often co-expressed with cytokeratin 8 (CK8). The CK18/CK8 heterodimer is the major cytokeratin pair found in simple epithelia such as that found in the liver, intestine and breast ducts. CK18 plays an important role in maintaining epithelial cell integrity.

This clone has also been demonstrated to cross-react with cytokeratin 19.

Product Details

Target Species
Human
Product Form
A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
Metal chelate affinity chromatogpraphy
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Recombinant human cytokeratin 18.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P05783
P08727
Entrez Gene
KRT18
KRT19
GO Terms
GO:0005515 protein binding
GO:0005198 structural molecule activity
GO:0007049 cell cycle
GO:0009653 anatomical structure morphogenesis
GO:0034451 centriolar satellite
GO:0043000 Golgi to plasma membrane CFTR protein transport
GO:0043066 negative regulation of apoptosis
GO:0044419 interspecies interaction between organisms
GO:0045095 keratin filament
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Cytokeratin 18/19 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Histology Positive Control Tissue
Human bladder
Western Blotting
HCA077 detects bands of approximately 42 and 45 kDa in HT-29 cell lysates
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

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