Cytokeratin 18/19 antibody | AbD03748
Human anti Human cytokeratin 18/19 antibody, clone AbD03748 recognizes human cytokeratin 18 (CK18), a 45 kDa type I intermediate filament protein expressed by epithelial cells. The intermediate filaments are expressed as heterodimers in epithelial tissues. CK18 is often co-expressed with cytokeratin 8 (CK8). The CK18/CK8 heterodimer is the major cytokeratin pair found in simple epithelia such as that found in the liver, intestine and breast ducts. CK18 plays an important role in maintaining epithelial cell integrity.
This clone has also been demonstrated to cross-react with cytokeratin 19.
This clone has also been demonstrated to cross-react with cytokeratin 19.
Product Details
- Target Species
- Human
- Product Form
- A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
- Preparation
- Metal chelate affinity chromatogpraphy
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Recombinant human cytokeratin 18.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P05783 Related reagents
- P08727 Related reagents
- Entrez Gene
- KRT18 Related reagents
- KRT19 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0005198 structural molecule activity
- GO:0007049 cell cycle
- GO:0009653 anatomical structure morphogenesis
- GO:0034451 centriolar satellite
- GO:0043000 Golgi to plasma membrane CFTR protein transport
- GO:0043066 negative regulation of apoptosis
- GO:0044419 interspecies interaction between organisms
- GO:0045095 keratin filament
- GO:0048471 perinuclear region of cytoplasm
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Cytokeratin 18/19 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Paraffin 1 | |||
| Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Histology Positive Control Tissue
- Human bladder
- Western Blotting
- HCA077 detects bands of approximately 42 and 45 kDa in HT-29 cell lysates
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | 0.1 mg | E WB | ||
| Mouse anti Histidine Tag:Biotin | MCA1396B | 0.1 mg | E WB | ||
| Mouse anti c-Myc:Alk.Phos. | MCA2200A | 0.1 mg | C E P WB* | ||
| Mouse anti c-Myc:Biotin | MCA2200B | 0.1 mg | C E P WB* | ||
| Mouse anti c-Myc:HRP | MCA2200P | 0.1 mg | C E P WB* | ||
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | 125 µl | E WB |
