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Cytokeratin 14 antibody

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Western Blot Validated
Anti Cytokeratin 14 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with cytokeratin 14 antibody (VMA00012) followed by detection with HRP conjugated Goat anti Human IgG F(ab')2 (1/2500, STAR126P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to cytokeratin 14 (molecular weight 52 kDa).

Human anti Cytokeratin 14

Product Type
PrecisionAb Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00012
Datasheet
WB
MSDS
100 µl loader

Human anti Human cytokeratin 14 antibody recognizes human cytokeratin 14 (CK14), a 472 amino acid, acidic type I intermediate filament. The intermediate filaments are expressed as heterodimers in epithelial tissues. CK14 is expressed in the basal compartment of all stratified squamous epithelia and is co-expressed with cytokeratin 5 (CK5).

Mutations in the cytokeratin 5 (KRT5) and cytokeratin 14 (KRT14) genes have been identified in patients with the skin disorder epidermolysis bullosa simplex (Coulombe et al. 1991) and in other epidermal conditions including Naegeli-Franceschetti-Jadassohn syndrome and dermatopathia pigmentosa reticularis (Lugassy et al. 2006).

Human anti Human cytokeratin 14 antibody recognizes human cytokeratin 14 as a band of ~52 kDa in HeLa cell lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti cytokeratin 14 antibody detects a band of approximately 52 kDa in HeLa cell lysates.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation
Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human cytokeratin 14

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P02533 Related reagents
Entrez Gene
KRT14 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005200 structural constituent of cytoskeleton
GO:0005634 nucleus
GO:0008544 epidermis development
GO:0031581 hemidesmosome assembly
GO:0045095 keratin filament
GO:0045110 intermediate filament bundle assembly
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of Cytokeratin 14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader

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