Cytokeratin 14 antibody

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Human anti Cytokeratin 14

Cytokeratin 14 antibody recognizes keratin, type I cytoskeletal 14, also known as cytokeratin-14, CK-14, keratin-14 or K14. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00012
Datasheet
WB
MSDS
100 µl loader

Human anti Human cytokeratin 14 antibody recognizes human cytokeratin 14 (CK14), a 472 amino acid, acidic type I intermediate filament. The intermediate filaments are expressed as heterodimers in epithelial tissues. CK14 is expressed in the basal compartment of all stratified squamous epithelia and is co-expressed with cytokeratin 5 (CK5).

Mutations in the cytokeratin 5 (KRT5) and cytokeratin 14 (KRT14) genes have been identified in patients with the skin disorder epidermolysis bullosa simplex (Coulombe et al. 1991) and in other epidermal conditions including Naegeli-Franceschetti-Jadassohn syndrome and dermatopathia pigmentosa reticularis (Lugassy et al. 2006).

Human anti Human cytokeratin 14 antibody recognizes human cytokeratin 14 as a band of ~52 kDa in HeLa cell lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti cytokeratin 14 antibody detects a band of approximately 52 kDa in HeLa cell lysates.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation
Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human cytokeratin 14
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P02533 Related reagents
Entrez Gene
KRT14 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005200 structural constituent of cytoskeleton
GO:0005634 nucleus
GO:0008544 epidermis development
GO:0031581 hemidesmosome assembly
GO:0045095 keratin filament
GO:0045110 intermediate filament bundle assembly
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of Cytokeratin 14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader
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