Cytokeratin 14 antibody | AbD03759
Lane 1: Western blot carried out using Human anti Human cytokeratin 14 antibody (VMA00012) at 0.5 µg/ml on Hela whole cell lysate. For detection, HRP conjugated Mouse anti-Histidine tag (MCA5995P ) was applied at 1/5000. Image was visualized on the Bio-Rad Chemidoc Imager.
Lane 1: western blot carried out using Human anti Human Cytokeratin 14 antibody (VMA00012) at 0.5 µg/ml on Hela whole cell lysate. For detection, HRP conjugated Goat anti-Human IgG F(ab')2 antibody (STAR126P ) was applied at 1/2500. Image was visualized on the Bio-Rad Chemidoc Imager
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WB ResetHuman anti Cytokeratin 14
- Product Type
- PrecisionAb Monoclonal
- Clone
- AbD03759
- Isotype
- HuCAL Fab bivalent
- Format
- Purified
- Specificity
- Cytokeratin 14
Antibody Quality and Performance Guarantee
| Human anti Human cytokeratin 14 antibody recognizes human cytokeratin 14 (CK14), a 472 amino acid, acidic type I intermediate filament. The intermediate filaments are expressed as heterodimers in epithelial tissues. CK14 is expressed in the basal compartment of all stratified squamous epithelia and is co-expressed with cytokeratin 5 (CK5). Mutations in the cytokeratin 5 (KRT5) and cytokeratin 14 (KRT14) genes have been identified in patients with the skin disorder epidermolysis bullosa simplex (Coulombe et al. 1991) and in other epidermal conditions including Naegeli-Franceschetti-Jadassohn syndrome and dermatopathia pigmentosa reticularis (Lugassy et al. 2006). Human anti Human cytokeratin 14 antibody recognizes human cytokeratin 14 as a band of ~52 kDa in HeLa cell lysates by western blotting under reducing conditions. |
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- Target Species
- Human
- Western Blotting
- Anti cytokeratin 14 antibody detects a band of approximately 52 kDa in HeLa cell lysates.
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
- Preparation
- Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human cytokeratin 14
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Immunohistology - Paraffin 1 | |
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| Western Blotting | |
1/1000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Penta Histidine Tag:HRP | ||||||
| Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Human IgG F(ab')2:HRP | ||||||
- UniProt
- P02533
- Entrez Gene
- KRT14
- GO Terms
- GO:0005515 protein binding
- GO:0005739 mitochondrion
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0005634 nucleus
- GO:0008544 epidermis development
- GO:0031581 hemidesmosome assembly
- GO:0045095 keratin filament
- GO:0045110 intermediate filament bundle assembly
- View More GO Terms
VMA00012
080714
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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