Cytokeratin 14 antibody | AbD03759


Product Details
- Target Species
- Human
- Product Form
- A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Recombinant human cytokeratin 14.
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P02533
- Entrez Gene
- KRT14
- GO Terms
- GO:0005515 protein binding
- GO:0005739 mitochondrion
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0005634 nucleus
- GO:0008544 epidermis development
- GO:0031581 hemidesmosome assembly
- GO:0045095 keratin filament
- GO:0045110 intermediate filament bundle assembly
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Cytokeratin 14 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunohistology - Paraffin 1 | 1/25 | 1/50 | |
Western Blotting | 1/1000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Histology Positive Control Tissue
- Human bladder
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg |
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Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
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Mouse anti c-Myc:Alk.Phos. | MCA2200A | C E P WB* | 0.1 mg |
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|
Mouse anti c-Myc:Biotin | MCA2200B | C E P WB* | 0.1 mg |
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|
Mouse anti c-Myc:HRP | MCA2200P | C E P WB* | 0.1 mg |
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|
Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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