Cytochrome P450 2B6 antibody | OTI3D5
Mouse anti Human cytochrome P450 2B6 antibody, clone OTI3D5 (VMA00171) used to assess Cyp2b levels in murine hepatic tissue lysates by western blotting.
Image caption:
Hepatic protein content of Cyp1A, Cyp2a, Cyp2b, Cyp2e1 and Cyp3a is independent on zeitgeber time and PGC-1α in mice. Values are mean ± standard error of the mean. * Different from ZT-2 within genotype (p <.05); § different from LOX within zeitgeber time (p < 0.05).
From: Yue F, Xing L, Wu S, Wei L, Zhou Z, Shi Y, Lam SM, Shui G, Xiang X, Russell R, Zhang D.
Constant light exposure alters gut microbiota and short-/medium-chain fatty acids and aggravates PCOS-like traits in HFD-fed rats.
Obesity (Silver Spring). 2022 Feb 6 [Epub ahead of print]..
doi: 10.1002/oby.23380.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Cytochrome P450 2B6
- Product Type
- PrecisionAb Monoclonal
- Clone
- OTI3D5
- Isotype
- IgG2a
- Format
- Purified
- Specificity
- Cytochrome P450 2B6
Antibody Quality and Performance Guarantee
| Mouse anti Human cytochrome P450 2B6 antibody, clone OTI3D5 recognizes cytochrome P450 2B6, also known as 1,4-cineole 2-exo-monooxygenase, cytochrome P450 IIB1, cytochrome P450, subfamily IIB (phenobarbital-inducible) and polypeptide 6. Encoded by the CYP2B6 gene, cytochrome P450 2B6 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for CYP2B6 have been described; however, it has not been resolved whether these transcripts are in fact produced by CYP2B6 or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q (provided by RefSeq, Jul 2008). Mouse anti Human cytochrome P450 2B6 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Anti cytochrome P450 2B6 detects a band of approximately 56 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human CYP2B6 (NP_000758) produced in HEK293T cells
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
References for Cytochrome P450 2B6 antibody
-
Yue, F. et al. (2022) Constant light exposure alters gut microbiota and short-/medium-chain fatty acids and aggravates PCOS-like traits in HFD-fed rats.
Obesity (Silver Spring). Feb 06 [Epub ahead of print].
- UniProt
- P20813
- Entrez Gene
- CYP2B6
- GO Terms
- GO:0009055 electron carrier activity
- GO:0005789 endoplasmic reticulum membrane
- GO:0005792 microsome
- GO:0055114 oxidation-reduction process
- GO:0006805 xenobiotic metabolic process
- GO:0008202 steroid metabolic process
- GO:0019825 oxygen binding
- GO:0020037 heme binding
- GO:0042180 cellular ketone metabolic process
- View More GO Terms
- GO:0042738 exogenous drug catabolic process
- GO:0070330 aromatase activity
VMA00171
240615
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