Cytochrome P450 2B6 antibody | OTI3D5

100% Secure


Mouse anti Cytochrome P450 2B6

Cytochrome P450 2B6 antibody recognizes cytochrome P450 2B6, also known as 1,4-cineole 2-exo-monooxygenase, cytochrome P450 IIB1 or cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6. This antibody has been extensively validated for western blotting using whole cell lysates.

Cytochrome P450 2B6 Antibody with Control Lysate

Cytochrome P450 2B6 antibody recognizes cytochrome P450 2B6, also known as 1,4-cineole 2-exo-monooxygenase, cytochrome P450 IIB1 or cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI3D5
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00171 WB 100 µl
VMA00171KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human cytochrome P450 2B6 antibody recognizes cytochrome P450 2B6, also known as 1,4-cineole 2-exo-monooxygenase, cytochrome P450 IIB1, cytochrome P450, subfamily IIB (phenobarbital-inducible) and polypeptide 6.

Encoded by the CYP2B6 gene, cytochrome P450 2B6 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for CYP2B6 have been described; however, it has not been resolved whether these transcripts are in fact produced by CYP2B6 or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q (provided by RefSeq, Jul 2008).

Mouse anti Human cytochrome P450 2B6 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human CYP2B6 (NP_000758) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P20813 Related reagents
Entrez Gene
CYP2B6 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0055114 oxidation-reduction process
GO:0006805 xenobiotic metabolic process
GO:0008202 steroid metabolic process
GO:0019825 oxygen binding
GO:0020037 heme binding
GO:0042180 cellular ketone metabolic process
GO:0042738 exogenous drug catabolic process
GO:0070330 aromatase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Cytochrome P450 2B6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti cytochrome P450 2B6 detects a band of approximately 56 kDa in Jurkat cell lysates.
Western Blotting
Anti cytochrome P450 2B6 detects a band of approximately 56 kDa in Jurkat cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB