Cytochrome C antibody | 2G8
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Mouse anti human Cytochrome C antibody, clone 2G8 recognises cytochrome c, originally termed Apaf-2, an evolutionarily conserved protein which acts as an essential mitochondrial electron carrier between respirable substrates and oxygen, during aerobic energy production.
A second major function for cytochrome c is its role as an intermediate in apoptosis, both through its association with inositol-3-phosphate receptor (IP3 receptor) which acts as an ER calcium release channel, and its association with apoptotic protease activating factor 1 (Apaf-1), resulting in the formation of the apoptosome protein complex which then recruits and activates pro-caspase 9 (Apaf-3).
Mouse anti human Cytochrome C antibody, clone 2G8 is reported to block the role of cytochrome c as a co-factor in the activation of caspase 9 (Neame et al. 1998) . Removal of sodium azide is recommended prior to use in functional assays.
A second major function for cytochrome c is its role as an intermediate in apoptosis, both through its association with inositol-3-phosphate receptor (IP3 receptor) which acts as an ER calcium release channel, and its association with apoptotic protease activating factor 1 (Apaf-1), resulting in the formation of the apoptosome protein complex which then recruits and activates pro-caspase 9 (Apaf-3).
Mouse anti human Cytochrome C antibody, clone 2G8 is reported to block the role of cytochrome c as a co-factor in the activation of caspase 9 (Neame et al. 1998) . Removal of sodium azide is recommended prior to use in functional assays.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human cytochrome c.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P99999
- Entrez Gene
- CYCS
- GO Terms
- GO:0000159 protein phosphatase type 2A complex
- GO:0005515 protein binding
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0005743 mitochondrial inner membrane
- GO:0005758 mitochondrial intermembrane space
- GO:0005759 mitochondrial matrix
- GO:0022904 respiratory electron transport chain
- GO:0006309 DNA fragmentation involved in apoptotic nuclear change
- GO:0006810 transport
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008635 activation of caspase activity by cytochrome c
- GO:0045155 electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity
- GO:0020037 heme binding
- GO:0070469 respiratory chain
- Regulatory
- For research purposes only
Applications of Cytochrome C antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry 1 | |||
| Western Blotting |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for Cytochrome C antibody
-
Neame, S.J. et al. (1998) Blocking cytochrome c activity within intact neurons inhibits apoptosis.
J Cell Biol. 142 (6): 1583-93.
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