Cystatin C antibody

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Mouse anti Cystatin C

Cystatin C antibody recognizes cystatin-C, also known as bA218C14.4 (cystatin C), CST3, cystatin 3, neuroendocrine basic polypeptide or post-gamma-globulin. This antibody has been extensively validated for western blotting using whole cell lysates.

Cystatin C Antibody with Control Lysate

Cystatin C antibody recognizes cystatin-C, also known as bA218C14.4 (cystatin C), CST3, cystatin 3, neuroendocrine basic polypeptide or post-gamma-globulin. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00105 WB 100 µl
VMA00105KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human cystatin C antibody recognizes cystatin C, also known as bA218C14.4, cystatin 3, cystatin-3, gamma-trace, neuroendocrine basic polypeptide and post-gamma-globulin.

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. The CST3 gene, which encodes Cystatin C, is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in CST3 has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease (provided by RefSeq, Jul 2008).

Mouse anti Human cystatin C antibody detects a band of 13 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Yeast-derived recombinant protein

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P01034 Related reagents
Entrez Gene
CST3 Related reagents
GO Terms
GO:0001540 beta-amyloid binding
GO:0002020 protease binding
GO:0006952 defense response
GO:0004869 cysteine-type endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0010703 negative regulation of histolysis
GO:0010711 negative regulation of collagen catabolic process
GO:0010716 negative regulation of extracellular matrix disassembly
GO:0043206 fibril organization
GO:0060311 negative regulation of elastin catabolic process
GO:0060313 negative regulation of blood vessel remodeling
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Cystatin C antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti cystatin C detects a band of approximately 13 kDa in A431 cell lysates
Western Blotting
Anti Cystatin C detects a band of approximately 13 kDa in A431 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB