Cyclin D1 antibody | CD1.1

Mouse anti Cyclin D1

Product Type
Monoclonal Antibody
Clone
CD1.1
Isotype
IgG1
Specificity
Cyclin D1

Product Code Applications Pack Size List Price Your Price Qty
MCA1756
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F * IP P * WB 0.1 mg loader
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loader
MCA1756T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * P * 10 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Cyclin D1 antibody, clone CD1.1 recognizes cyclin D1, a 295 amino acid, ~36 kDa nuclear protein which belongs to the highly conserved cyclin family. D type cyclins are predominantly expressed in the G1 phase of the cell cycle.

Cyclin D1 study is of interest in various forms of malignancy and over expression has been linked to poor prognosis (Bartkova et al. 1995).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA1756: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1756T: Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Human Cyclin D1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse Sp-2 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 Neat
Immunohistology - Frozen
Immunohistology - Paraffin 2 1/1000 1/10000
Immunoprecipitation
Western Blotting 1/500
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10°l of the suggested working dilution to label 1x106 cells in 100°l
Histology Positive Control Tissue
Small intestine, colon

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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for Cyclin D1 antibody

  1. Wykoff, C.C. et al. (2004) Gene array of VHL mutation and hypoxia shows novel hypoxia-induced genes and that cyclin D1 is a VHL target gene.
    Br J Cancer. 90 (6): 1235-43.
  2. Zeldich, E. et al. (2012) Enamel matrix derivative stimulates human gingival fibroblast proliferation via ERK.
    J Dent Res. 86: 41-6.
  3. Jovanovic, I.P. et al. (2014) Clinical significance of Cyclin D1, FGF3 and p21 protein expression in laryngeal squamous cell carcinoma.
    J BUON. 19 (4): 944-52.

Western Blotting

RRID
AB_2084955
UniProt
P24385
Entrez Gene
CCND1
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000307 cyclin-dependent protein kinase holoenzyme complex
GO:0001934 positive regulation of protein phosphorylation
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0042493 response to drug
GO:0019901 protein kinase binding
GO:0031571 mitotic cell cycle G1/S transition DNA damage checkpoint
GO:0045737 positive regulation of cyclin-dependent protein kinase activity
GO:0051301 cell division
GO:0070141 response to UV-A
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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