Cyclin D1 antibody | CD1.1

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry 1			Neat
Immunohistology - Frozen
Immunohistology - Paraffin 2		1/1000	1/10000
Immunoprecipitation
Western Blotting			1/500

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
2. ²This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10°l of the suggested working dilution to label 1x10⁶ cells in 100°l

Histology Positive Control Tissue

Small intestine, colon

References for Cyclin D1 antibody

1. Wykoff, C.C. et al. (2004) Gene array of VHL mutation and hypoxia shows novel hypoxia-induced genes and that cyclin D1 is a VHL target gene.
   Br J Cancer. 90 (6): 1235-43.
2. Zeldich, E. et al. (2012) Enamel matrix derivative stimulates human gingival fibroblast proliferation via ERK.
   J Dent Res. 86: 41-6.
3. Jovanovic, I.P. et al. (2014) Clinical significance of Cyclin D1, FGF3 and p21 protein expression in laryngeal squamous cell carcinoma.
   J BUON. 19 (4): 944-52.

Western Blotting

RRID

AB_2084955

UniProt

P24385

Entrez Gene

CCND1

GO Terms

GO:0000080 G1 phase of mitotic cell cycle

GO:0000082 G1/S transition of mitotic cell cycle

GO:0000084 S phase of mitotic cell cycle

GO:0000307 cyclin-dependent protein kinase holoenzyme complex

GO:0001934 positive regulation of protein phosphorylation

GO:0005829 cytosol

GO:0005654 nucleoplasm

GO:0042493 response to drug

GO:0019901 protein kinase binding

View More GO Terms

GO:0031571 mitotic cell cycle G1/S transition DNA damage checkpoint

GO:0045737 positive regulation of cyclin-dependent protein kinase activity

GO:0051301 cell division

GO:0070141 response to UV-A

MCA1756

151777 270612 270612R

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MCA1756T

153064

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