Cyclin B1 antibody | EF02/2D4-1

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Anti Cyclin B1 Antibody, clone EF02/2D4-1 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Cyclin B1 Antibody, clone EF02/2D4-1 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CCNB1 antibody (1/1,000, **VMA00732**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to CCNB1 (molecular weight 56 kDa).

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Mouse anti Cyclin B1

Product Type: PrecisionAb Monoclonal
Clone: EF02/2D4-1
Isotype: IgG2b
Format: Purified
Specificity: Cyclin B1

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Mouse anti Human cyclin B1 antibody recognizes the G2/mitotic-specific cyclin-B1, also known as CCNB1.

Progression through the cell cycle is controlled by timely interaction between cyclins and cyclin-dependent kinases (CDKs), and cyclin B1 is required for cell division (Strauss et al. 2018). The complex between cyclin B1 and Cdk1 regulates entry into mitosis after G2 phase, synchronizing the breakdown of the nuclear envelope and separation of centrosomes (Wang et al. 2014). The protein p21 can prevent untimely entry into mitosis by inhibiting cyclin B1, holding the cyclin B1/Cdk1 complex in the nucleus and activating degradation of cyclin B1 in response to DNA damage (Jang et al. 2016). Cyclin B1 is overexpressed in numerous malignant tumors, and is significantly associated with poor overall survival of patients with solid tumors. This finding has suggested that cyclin B1 could be a prognostic marker for these cancer types (Ye et al. 2017).

Target Species: Human
Western Blotting: Mouse anti cyclin B1 detects a band of approximately 56 kDa in K562 cell lysates
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli--derived recombinant protein of amino acids 1-433 of human cyclin B1
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P14635
Entrez Gene: CCNB1
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0000086 G2/M transition of mitotic cell cycle; GO:0000236 mitotic prometaphase; GO:0000922 spindle pole; GO:0000942 condensed nuclear chromosome outer kinetochore; GO:0005829 cytosol; GO:0005654 nucleoplasm; GO:0005815 microtubule organizing center; GO:0007080 mitotic metaphase plate congression; View More GO Terms; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0043148 mitotic spindle stabilization; GO:0045931 positive regulation of mitotic cell cycle; GO:0051301 cell division; GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0051987 positive regulation of attachment of spindle microtubules to kinetochore; GO:0071174 mitotic cell cycle spindle checkpoint