Cyclin B1 antibody | EF02/2D4-1

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Mouse anti Cyclin B1

Product Type
PrecisionAb Monoclonal
Clone
EF02/2D4-1
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00732
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human cyclin B1 antibody recognizes the G2/mitotic-specific cyclin-B1, also known as CCNB1.

Progression through the cell cycle is controlled by timely interaction between cyclins and cyclin-dependent kinases (CDKs), and cyclin B1 is required for cell division (Strauss et al. 2018). The complex between cyclin B1 and Cdk1 regulates entry into mitosis after G2 phase, synchronizing the breakdown of the nuclear envelope and separation of centrosomes (Wang et al. 2014). The protein p21 can prevent untimely entry into mitosis by inhibiting cyclin B1, holding the cyclin B1/Cdk1 complex in the nucleus and activating degradation of cyclin B1 in response to DNA damage (Jang et al. 2016). Cyclin B1 is overexpressed in numerous malignant tumors, and is significantly associated with poor overall survival of patients with solid tumors. This finding has suggested that cyclin B1 could be a prognostic marker for these cancer types (Ye et al. 2017).

Product Details

Target Species
Human
Western Blotting
Mouse anti cyclin B1 detects a band of approximately 56 kDa in K562 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli--derived recombinant protein of amino acids 1-433 of human cyclin B1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P14635
Entrez Gene
CCNB1
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000236 mitotic prometaphase
GO:0000922 spindle pole
GO:0000942 condensed nuclear chromosome outer kinetochore
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0005815 microtubule organizing center
GO:0007080 mitotic metaphase plate congression
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0043148 mitotic spindle stabilization
GO:0045931 positive regulation of mitotic cell cycle
GO:0051301 cell division
GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0051987 positive regulation of attachment of spindle microtubules to kinetochore
GO:0071174 mitotic cell cycle spindle checkpoint
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Cyclin B1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml