CXCL7 antibody | C06-A43

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Mouse anti Human CXCL7:Biotin

Product Type
Monoclonal Antibody
Clone
C06-A43
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6225B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human CXCL7 antibody, clone C11-5G10, recognizes C-X-C motif chemokine 7 (CXCL7), also known as Platelet Basic Protein, a platelet derived growth factor belonging to the C-X-C subfamily of chemokines. Expression of CXCL7 is largely restricted to platelets where it is produced as an inactive precursor. Upon tissue injury or infection, CXCL7 is one of the first chemokines to appear at the focal sites to mediate neutrophil recruitment after it has been modified by a series of cleavage and activation steps. CXCL7 mediates neutrophil recruitment through binding of its receptors CXCR1 or CXCR2.

CXCL7 has been noted as playing important roles in tumorigenesis and has been associated with the proliferation and metastasis of a range of tumor types (Brown et al. 2017). As such, there have been a range of studies performed testing the use of serum CXCL7 as a diagnostic biomarker in colorectal cancer, lung cancer and renal cell carcinoma samples (Li et al. 2019, Kinouchi et al. 2017, Du et al. 2018). These studies have suggested that serum CXCL7 may be a sensitive and specific biomarker for the diagnosis of these cancer types.

The biotinylated Mouse anti Human CXCL7 antibody, clone C06-4A3 (MCA6225B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CXCL7 antibody, clone C11-5G10 (MCA6224GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length CXCL7 (Ala59-Asp128)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P02775
Entrez Gene
PPBP
GO Terms
GO:0002576 platelet degranulation
GO:0042742 defense response to bacterium
GO:0005355 glucose transmembrane transporter activity
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006955 immune response
GO:0008009 chemokine activity
GO:0008083 growth factor activity
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0051781 positive regulation of cell division
Regulatory
For research purposes only

Applications of CXCL7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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