CREB1 antibody | EF03/2E2

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Anti CREB1 Antibody, clone EF03/2E2 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti CREB1 Antibody, clone EF03/2E2 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti CREB1 Antibody, clone EF03/2E2 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CREB1 antibody (1/1,000, **VMA00876**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to CREB1 (molecular weight 40 kDa)

Anti CREB1 Antibody, clone EF03/2E2 (PrecisionAb Monoclonal Antibody) gallery image 2 — HeLa cells stained with Mouse anti CREB1 antibody (**VMA00876**) at a 1/500 dilution followed by detection with an Alexa Fluor 488 conjugated Goat anti Mouse IgG (H/L): antibody (green) and PureBlu™ DAPI (1351303) (blue) as a nuclear counterstain.

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Mouse anti CREB1

Product Type: PrecisionAb Monoclonal
Clone: EF03/2E2
Isotype: IgG1
Format: Purified
Specificity: CREB1

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00876 Datasheet Datasheet SDS SDS	IF WB	100 µl		Log in	Get Quote
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Mouse anti CREB1 antibody recognizes cyclic AMP-responsive element-binding protein 1.

The protein CREB1 is a transcription factor that regulates transcription of genes involved with DNA repair and metabolism through phosphorylation and dephosphorylation. Targets of activated CREB1 include cyclins, Bcl-2 family members, and Egr-1 (Yan et al. 2018). The gene encoding CREB1 is a proto-oncogene, and CREB1 overexpression has been reported in a variety of cancers including gastric, colorectal, ovarian, bladder, and breast cancers (Guo et al. 2018). The cAMP/CREB1 pathway is involved with resistance of cancer cells to certain drug treatments. For example, CREB1 appears to confer tamoxifen resistance to breast cancer (Zhu et al. 2016). Activation of CREB1 and genes downstream of CREB1 also contribute to epilepsy, and epileptic mice with decreased CREB1 levels show around a 50% reduction in spontaneous seizures (Wang et al. 2016).

Target Species: Human
Western Blotting: Mouse anti CREB1 antibody detects a band of approximately 40 kDa in HEK293 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-341 of human CREB1
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P16220
Entrez Gene: CREB1
GO Terms: GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0003712 transcription cofactor activity; GO:0006468 protein phosphorylation; GO:0007202 activation of phospholipase C activity; GO:0007268 synaptic transmission; GO:0007411 axon guidance; GO:0008063 Toll signaling pathway; GO:0010033 response to organic substance; View More GO Terms; GO:0048015 phosphatidylinositol-mediated signaling; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0044419 interspecies interaction between organisms; GO:0045087 innate immune response; GO:0046983 protein dimerization activity; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051403 stress-activated MAPK cascade