Creatine Phosphokinase antibody
Mouse anti Creatine Phosphokinase
Mouse anti human creatine phosphokinase antibody recognizes the enzyme creatine phosphokinase, also known as creatine kinase, phospho-creatine kinase, CK or CPK. This antibody has been extensively validated for western blotting using whole cell lysates.
Creatine Phosphokinase Antibody with Control Lysate
Mouse anti human creatine phosphokinase antibody recognizes the enzyme creatine phosphokinase, also known as creatine kinase, phospho-creatine kinase, CK or CPK. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00404 | WB | 100 µl | ||||
| VMA00404KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human creatine phosphokinase antibody recognizes creatine phosphokinase which reversibly catalyzes the transfer of phosphate between ATP and creatine to produce creatine phosphate. In cells, the cytosolic creatine phosphokinase enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes with homodimers or heterodimers of the M and B types: CKMM or CKBB and CKMB respectively. CKBB is found in the brain while CKMB occurs in heart tissue (provided by RefSeq, Jul 2008).
Mouse anti Human creatine phosphokinase antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human creatine phosphokinase antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti creatine phosphokinase detects a band of approximately 42 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E.coli-derived recombinant human creatine phosphokinase
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P12277 Related reagents
- P06732 Related reagents
- Entrez Gene
- CKM Related reagents
- CKB Related reagents
- GO Terms
- GO:0004111 creatine kinase activity
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0006600 creatine metabolic process
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Creatine Phosphokinase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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