Creatine Phosphokinase antibody

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Mouse anti Creatine Phosphokinase

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00404
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human creatine phosphokinase antibody recognizes creatine phosphokinase which reversibly catalyzes the transfer of phosphate between ATP and creatine to produce creatine phosphate. In cells, the cytosolic creatine phosphokinase enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes with homodimers or heterodimers of the M and B types: CKMM or CKBB and CKMB respectively. CKBB is found in the brain while CKMB occurs in heart tissue (provided by RefSeq, Jul 2008).

Mouse anti Human creatine phosphokinase antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti creatine phosphokinase detects a band of approximately 42 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E.coli-derived recombinant human creatine phosphokinase

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P12277 Related reagents
P06732 Related reagents
Entrez Gene
CKM Related reagents
CKB Related reagents
GO Terms
GO:0004111 creatine kinase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006600 creatine metabolic process
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Creatine Phosphokinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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