Creatine Phosphokinase antibody
Mouse anti Human creatine phosphokinase antibody recognizes creatine phosphokinase which reversibly catalyzes the transfer of phosphate between ATP and creatine to produce creatine phosphate. In cells, the cytosolic creatine phosphokinase enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes with homodimers or heterodimers of the M and B types: CKMM or CKBB and CKMB respectively. CKBB is found in the brain while CKMB occurs in heart tissue (provided by RefSeq, Jul 2008).
Mouse anti Human creatine phosphokinase antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human creatine phosphokinase antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti creatine phosphokinase detects a band of approximately 42 kDa in HEK293 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- E.coli-derived recombinant human creatine phosphokinase
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P12277
- P06732
- Entrez Gene
- CKM
- CKB
- GO Terms
- GO:0004111 creatine kinase activity
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0006600 creatine metabolic process
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Creatine Phosphokinase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
