Creatine Phosphokinase antibody

Mouse anti Creatine Phosphokinase

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
Creatine Phosphokinase

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VMA00335
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Mouse anti Human creatine phosphokinase antibody recognizes the creatine kinase B-type, also known as creatine kinase B chain, creatine kinase B-type, creatine kinase-B, epididymis luminal protein 211 and epididymis secretory protein Li 29.

Encoded by the CKB gene, creatine kinase B-type is a cytoplasmic enzyme involved in energy homeostasis which reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. Creatine kinase B-type is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of CKB has been characterized (provided by RefSeq, Jul 2008).

Mouse anti Human creatine phosphokinase antibody detects a band of 43 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti creatine phosphokinase detects a band of approximately 43 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant human creatine phosphokinase
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P12277
Entrez Gene
CKB
GO Terms
GO:0004111 creatine kinase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006600 creatine metabolic process