Creatine Phosphokinase antibody | E05/10G2

Mouse anti Creatine Phosphokinase

Product Type
PrecisionAb Monoclonal
Clone
E05/10G2
Isotype
IgG1
Format
Purified
Specificity
Creatine Phosphokinase

Product Code Applications Pack Size List Price Your Price Qty
VMA00404
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human creatine phosphokinase antibody, clone E05/10G2 recognizes creatine phosphokinase which reversibly catalyzes the transfer of phosphate between ATP and creatine to produce creatine phosphate. In cells, the cytosolic creatine phosphokinase enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes with homodimers or heterodimers of the M and B types: CKMM or CKBB and CKMB respectively. CKBB is found in the brain while CKMB occurs in heart tissue (provided by RefSeq, Jul 2008).

Mouse anti Human creatine phosphokinase antibody, clone E05/10G2 detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti creatine phosphokinase detects a band of approximately 42 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E.coli-derived recombinant human creatine phosphokinase
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P12277
P06732
Entrez Gene
CKM
CKB
GO Terms
GO:0004111 creatine kinase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006600 creatine metabolic process